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VEGFA Mouse Monoclonal Antibody [Clone ID: OTI4E3]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
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Applications:

IF, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Human recombinant protein fragment corresponding to amino acids 27-233 of human VEGFA (NP_003367) produced in E.coli.

Alternative Names:

MVCD1; VEGF; VPF

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Product Description:

VEGFA (VEGF) mouse monoclonal antibody, clone OTI4E3 (formerly 4E3)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGF (RC223789, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGF.
HEK293T cells were transfected with the
HEK293T cells were transfected with the overexpression plasmids of 9 VEGF isoforms (from left to right:VEGF206, RC223789; VEGF189, RC229706; L-VEGF165, RC223884; VEGF165, RC229662; L-VEGF121, RC222129; VEGF iso18, RC229874; VEGF183, RC229686; VEGF145, RC231952; L-VEGF189, RC224244) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-flag antibody (TA180144, 1:1000) or anti-VEGFA mouse monoclonal antibody. (TA500289, 1:500)
Western blot analysis of extracts (10ug)
Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-VEGF monoclonal antibody (1:200).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500289)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500289)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500289)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500289)
Anti-VEGF mouse monoclonal antibody (TA5
Anti-VEGF mouse monoclonal antibody (TA500289) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VEGF (RC223789).
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