www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: OTI4D12]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£533.00 Shipping is calculated in checkout
Applications:

IF, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Dog; Monkey; Mouse; Rat

Antibody Isotype:

IgG2a

Antibody Type:

Monoclonal

Immunogen:

Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.

Alternative Names:

CARD2; CK-8; CK8; CYK8; K2C8; K8; KO

image 0
image 1
image 2
Product Description:

CK8 (Keratin 8) mouse monoclonal antibody, clone OTI4D12 (formerly 4D12)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8. Positive lysates LY419425 (100ug) and LC419425 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Equivalent amounts of cell lysates (10 u
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and KRT8-Knockout Hela cells (KO, Cat# LC810123) were separated by SDS-PAGE and immunoblotted with anti-KRT8 monoclonal antibody TA500216, (1:1000). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500216)
Anti-KRT8 mouse monoclonal antibody (TA5
Anti-KRT8 mouse monoclonal antibody (TA500216) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8 (RC209570).
Immunofluorescent staining of A549 cells
Immunofluorescent staining of A549 cells using anti-KRT8 mouse monoclonal antibody (TA500216).
Details Cat number & supplier Size Price
Goat anti-Mouse IgG Heavy and Light Chain Antibody HRP Conjugated A90-116P · Bethyl Laboratories, Inc.
A90-116P
Bethyl Laboratories, Inc.
1 mg £91.20
1 mg
view
Goat anti-Mouse IgG Heavy and Light Chain Cross-Adsorbed Antibody HRP Conjugated A90-216P · Bethyl Laboratories, Inc.
A90-216P
Bethyl Laboratories, Inc.
0.5 mg £99.20
0.5 mg
view