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B Raf (BRAF) Mouse Monoclonal Antibody [Clone ID: OTI4C11]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
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Applications:

ELISA, IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Dog; Mouse; Rat

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full-length protein expressed in 293T cell transfected with human BRAF expression vector

Alternative Names:

B-raf; B-RAF1; BRAF1; NS7; RAFB1

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Product Description:

Anti-BRAF mouse monoclonal antibody, clone OTI4C11 (formerly 4C11)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF. Positive lysates LY401382 (100ug) and LC401382 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-BRAFmonoclonal antibody.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 4 tissue lysates by using anti-BRAF monoclonal antibody (1:500).
Equivalent amounts of cell lysates (10 u
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and BRAF-Knockout HeLa cells (KO, Cat# LC835315) were separated by SDS-PAGE and immunoblotted with anti-BRAF monoclonal antibody TA500462 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Standard curve for ELISA analysis with B
Standard curve for ELISA analysis with BRAF recombinant protein (dilution range from 0.8ng/ml to 600ng/ml) using BRAF Capture Antibody (Cat# TA500429/TA500462) at 5ug/ml and HRP conjugated BRAF Detection mAb (Cat# TA500438) at 0.03ug/ml.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-BRAF mouse monoclonal antibody. (TA500462)
Immunoprecipitation (IP) of BRAF by usin
Immunoprecipitation (IP) of BRAF by using TrueMab monoclonal anti-BRAF antibodies (Negative control: IP without adding anti-BRAF antibody.). For each experiment, 500ul of DDK tagged BRAF overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-BRAF antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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