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MADM (NRBP1) Mouse Monoclonal Antibody [Clone ID: OTI7C5]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
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Applications:

FC, IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Dog; Rat; Monkey; Mouse

Antibody Isotype:

IgG2b

Antibody Type:

Monoclonal

Immunogen:

Full-length protein expressed in 293T cell transfected with human NRBP1 expression vector

Alternative Names:

BCON3; MADM; MUDPNP; NRBP

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Product Description:

Anti-NRBP1 (MADM) mouse monoclonal antibody, clone OTI7C5 (formerly 7C5)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRBP1 (RC200107, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRBP1. Positive lysates LY415614 (100ug) and LC415614 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NRBP1 monoclonal antibody.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NRBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500442)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-NRBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500442)
Immunoprecipitation (IP) of NRBP1 by usi
Immunoprecipitation (IP) of NRBP1 by using TrueMab monoclonal anti-NRBP1 antibodies (Negative control: IP without adding anti-NRBP1 antibody.). For each experiment, 500ul of DDK tagged NRBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NRBP1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC
HEK293T cells transfected with either RC200107 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NRBP1 antibody (TA500442), and then analyzed by flow cytometry.
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