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B Raf (BRAF) Mouse Monoclonal Antibody [Clone ID: OTI4A5]

Catalogue number:
100 µl _$$_
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Antibody Host:


Species Reactivity:

Human; Mouse; Rat

Antibody Isotype:


Antibody Type:



Full-length protein expressed in 293T cell transfected with human BRAF expression vector

Alternative Names:

B-raf; B-RAF1; BRAF1; NS7; RAFB1

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Product Description:

Anti-BRAF mouse monoclonal antibody, clone OTI4A5 (formerly 4A5)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (RC211013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF (1:500).
Standard curve for ELISA analysis with B
Standard curve for ELISA analysis with BRAF recombinant protein (dilution range from 0.8ng/ml to 600ng/ml) using BRAF Capture Antibody (Cat# TA500429/TA500462) at 5ug/ml and HRP conjugated BRAF Detection mAb (Cat# TA500438) at 0.03ug/ml.
Immunoprecipitation (IP) of BRAF by usin
Immunoprecipitation (IP) of BRAF by using TrueMab monoclonal anti-BRAF antibodies (Negative control: IP without adding anti-BRAF antibody.). For each experiment, 500ul of DDK tagged BRAF overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-BRAF antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Details Cat number & supplier Size Price
Goat anti-Mouse IgG Heavy and Light Chain Cross-Adsorbed Antibody HRP Conjugated A90-216P · Bethyl Laboratories, Inc.
Bethyl Laboratories, Inc.
0.5 mg £99.20
0.5 mg