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Cytokeratin 8 (KRT8) Rabbit Monoclonal Antibody [Clone ID: E431-2]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
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Applications:

IF, IHC

Antibody Host:

Rabbit

Species Reactivity:

Mouse; Rat; Human

Antibody Isotype:

IgG

Antibody Type:

Monoclonal

Immunogen:

A synthetic phospho-peptide corresponding to residues surrounding Ser73 of human Cytokeratin 8 was used as immunogen. The antibody only detects Kerati

Alternative Names:

CARD2; CK-8; CK8; CYK8; K2C8; K8; KO

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Product Description:

Rabbit Monoclonal Antibody against KRT8 (Clone E431-2)

Storage Temperature:

Store at -20°C as received.

Immunohistochemistry (Formalin/PFA-fixed
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 8 (phospho S73) antibody [E431-2]; IHC-P analysis (with antigen retrieval) of liver carcinoma using anti-Phospho-Keratin 8 diluted 1/500.
IHC of Mouse lung adenoma, staining Cyto
IHC of Mouse lung adenoma, staining Cytokeratin 8 (phospho S73) with TA300410.Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/1000 in PBS) for 8 hours at 4°C. A biotin-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence
Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 (phospho S73) antibody [E431-2]; Immunofluorescent staining of A431 cells using anti-Phospho-Keratin 8 diluted 1/250.
Immunocytochemistry/ Immunofluorescence
Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 (phospho S73) antibody [E431-2]; Ab32579 staining cells from a human pancreatic cell line by ICC/IF. The cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 22°C. Ab32579 was diluted 1/25 and incubated with the sample for 1 hour at 22°C. An Alexa Fluor#174; 488 conjugated donkey anti-rabbit antibody was used as the secondary.
Details Cat number & supplier Size Price
Goat anti-Rabbit IgG Heavy and Light Chain Highly Cross-Adsorbed Antibody HRP Conjugated A120-501P · Bethyl Laboratories, Inc.
A120-501P
Bethyl Laboratories, Inc.
1 mg £155.20
1 mg
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