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SOX2 Mouse Monoclonal Antibody

Catalogue number:
Supplier:
Size:
400 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

FC, IF, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

IgG1, kappa

Antibody Type:

Monoclonal

Immunogen:

SOX2 recombinant protein is used to produce this monoclonal antibody.

Alternative Names:

ANOP3; MCOPS3

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Product Description:

Mouse Monoclonal Antibody against SOX2

Storage Temperature:

Store at -20°C as received.

Western blot analysis of SOX2 Antibody (
Western blot analysis of SOX2 Antibody (Cat.#TA302025) by SOX2 recombinant protein. SOX2 (arrow) was detected using the purified Mab.
Western blot analysis of SOX2 (arrow) us
Western blot analysis of SOX2 (arrow) using mouse monoclonal SOX2 antibody (Cat.#TA302025). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies)
Formalin-fixed and paraffin-embedded hum
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody (Cat.#TA302025), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF image of A549 cell stained with SOX2
IF image of A549 cell stained with SOX2 Antibody (Cat#TA302025/SG110310AA).A549 cells were incubated with SOX2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml).SOX2 immunoreactivity is localized to Nucleus significantly.
Fluorescent confocal image of SY5Y cells
Fluorescent confocal image of SY5Y cells stained with SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA302025 SOX2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Note the highly specific localization of the SOX2 mainly to the nucleus.
Flow cytometric analysis of NCI-H460 cel
Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
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