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BNIP3L Rabbit Polyclonal Antibody

Catalogue number:
Supplier:
Size:
400 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

IF, IHC, IP, WB

Antibody Host:

Rabbit

Species Reactivity:

Human; Mouse

Antibody Isotype:

Ig

Antibody Type:

Polyclonal

Immunogen:

This BNIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-187 amino acids from human BNIP3.

Alternative Names:

BNIP3a; NIX

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Product Description:

Rabbit Polyclonal Antibody against BNIP3L

Storage Temperature:

Store at -20°C as received.

The anti-NIP3 BH3 domain Pab (Cat. #TA30
The anti-NIP3 BH3 domain Pab (Cat. #TA302077) is used in Western blot to detect NIP3 BH3 in Ramos cell lysate (lane 1) and in mouse brain tissue lysate (lane 2).
Formalin-fixed and paraffin-embedded hum
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Freshly isolated mouse hepatocytes plate
Freshly isolated mouse hepatocytes plated on coverslips (2 x105 cells/22-mm glass coverslip) were cultured under normoxic conditions for 6 hr. The cells were then fixed in 2% paraformaldehyde in PBS for 1 hr, and processed for confocal immunofluorescence (red: F-actin, blue: ATP-synthase, green: BNIP3). Fluorescence labeling of BNIP3 accomplished with anti-BNIP3 antibody Cat # TA302077. Data courtesy of Ruben Zamora, University of Pittsburgh.
IF image of HepG2 cells stained with BNI
IF image of HepG2 cells stained with BNIP3 (BH3 Domain Specific) antibody. HepG2 cells were incubated with TA302077 BNIP3 (BH3 Domain Specific) primary antibody (1:500, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells.
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