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BAD Rabbit Monoclonal Antibody [Clone ID: Y208]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

FC, IF, IHC, WB

Antibody Host:

Rabbit

Species Reactivity:

Mouse; Rat; Dog; Human

Antibody Isotype:

IgG

Antibody Type:

Monoclonal

Immunogen:

A synthetic peptide corresponding to residues in the N-term of human Bad was used as immunogen. The antibody does not cross-react with other Bcl-2 mem

Alternative Names:

BBC2; BCL2L8

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Product Description:

Rabbit Monoclonal Antibody against BAD (Clone Y208)

Storage Temperature:

Store at -20°C as received.

Western blot - Bad antibody [Y208]; Anti
Western blot - Bad antibody [Y208]; Anti-Bad [Y208] antibody at 1/5000 dilution + Hela cell lysate.Predicted band size : 18 kDa.Observed band size : 23 kDa.
Immunohistochemistry (Paraffin-embedded
Immunohistochemistry (Paraffin-embedded sections) - Bad antibody [Y208]; Immunohistochemical staining of paraffin-embedded human lymphoma using TA300475 at 1/100 dilution.
ICC/IF image of TA300475 stained MCF7 ce
ICC/IF image of TA300475 stained MCF7 cells. The cells were incubated with the antibody overnight at 4. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry-Bad antibody (TA300475);
Flow Cytometry-Bad antibody (TA300475); Overlay histogram showing MCF-7 cells stained with TA300475 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. This antibody gave a decreased signal in MCF-7 cells under the same conditions.
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