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Caspase 3 (CASP3) Rabbit Monoclonal Antibody [Clone ID: E83-103]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
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£680.00 Shipping is calculated in checkout
Applications:

FC, IF, IHC, WB

Antibody Host:

Rabbit

Species Reactivity:

Mouse; Human (Does not react with: Rat)

Antibody Isotype:

IgG

Antibody Type:

Monoclonal

Immunogen:

A synthetic peptide corresponding to residues following Ser29 of human caspase-3 (N-terminus of p17 subunit) was used as immunogen. This antibody only

Alternative Names:

CPP32; CPP32B; SCA-1

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Product Description:

Rabbit Monoclonal Antibody against CASP3 (Clone E83-103)

Storage Temperature:

Store at -20°C as received.

Western blot - pro Caspase 3 antibody [Y
Western blot - pro Caspase 3 antibody [Y83-103]; All lanes : Anti-pro Caspase 3 antibody [E83-103] at 1/10000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Jurkat cell lysate + Camptothecin.Predicted band size : 31 kDa.Observed band size : 35 kDa.
Immunohistochemistry (Paraffin-embedded
Immunohistochemistry (Paraffin-embedded sections) - pro Caspase 3 antibody [Y83-103]; Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma TA300422 at 1/250 dilution.
ICC/IF image of TA300422 stained HeLa ce
ICC/IF image of TA300422 stained HeLa cells. The cells were incubated with the antibody overnight at 4. The secondary antibody (green) was, DyLight 488 goat anti-rabbit IgG (H+L) used at 1:250. for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry-Anti-pro Caspase 3 antibo
Flow Cytometry-Anti-pro Caspase 3 antibody (TA300422); Overlay histogram showing Jurkat cells stained with TA300422 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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