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Kindlin 2 (FERMT2) Mouse Monoclonal Antibody [Clone ID: OTI9E4]

Catalogue number:
Supplier:
Size:
100 µl
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
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Applications:

IF, IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Dog; Rat; Monkey; Mouse

Antibody Isotype:

IgG2a

Antibody Type:

Monoclonal

Immunogen:

Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector

Alternative Names:

KIND2; mig-2; MIG2; PLEKHC1; UNC112; UNC112B

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Product Description:

Anti-FERMT2 mouse monoclonal antibody, clone OTI9E4 (formerly 9E4)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2. Positive lysates LY416377 (100ug) and LC416377 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
Equivalent amounts of cell lysates (10 u
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and FERMT2-Knockout HeLa cells (KO, Cat# LC812552) were separated by SDS-PAGE and immunoblotted with anti-FERMT2 monoclonal antibody TA500504 (1:1000`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500504)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500504)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500504)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500504)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500504)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500504)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500504)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500504)
Immunofluorescent staining of HepG2 cell
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (TA500504).
Immunoprecipitation (IP) of FERMT2 by us
Immunoprecipitation (IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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