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ERK2 (MAPK1) Mouse Monoclonal Antibody [Clone ID: OTI6F8]

Catalogue number:
Supplier:
Size:
100 µl
Product is available in:
  • UK
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Applications:

FC, IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Dog; Rat; Monkey; Mouse

Antibody Isotype:

IgG2a

Antibody Type:

Monoclonal

Immunogen:

Full length human recombinant protein of human MAPK1(NP_620407) produced in HEK293T cell.

Alternative Names:

ERK; ERK-2; ERK2; ERT1; MAPK2; NS13; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; PRKM1; PRKM2

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Product Description:

Anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI6F8 (formerly 6F8)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (RC204703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1. Positive lysates LY408481 (100ug) and LC408481 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAPK1 monoclonal antibody.
Western blot analysis of extracts (10ug)
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-MAPK1 monoclonal antibody (1:200).
Equivalent amounts of cell lysates (10 u
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and MAPK1-Knockout Hela cells (KO, Cat# LC810185) were separated by SDS-PAGE and immunoblotted with anti-MAPK1 monoclonal antibody TA500468. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500468)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500468)
Immunoprecipitation (IP) of MAPK1 by usi
Immunoprecipitation (IP) of MAPK1 by using TrueMab monoclonal anti-MAPK1 antibodies (Negative control: IP without adding anti-MAPK1 antibody.). For each experiment, 500ul of DDK tagged MAPK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAPK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC
HEK293T cells transfected with either RC204703 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK1 antibody (TA500468), and then analyzed by flow cytometry.
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