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LDHA Mouse Monoclonal Antibody [Clone ID: OTI2D11]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
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Applications:

IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Mouse

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full length human recombinant protein of human LDHA (NP_005557) produced in HEK293T cell.

Alternative Names:

GSD11; HEL-S-133P; LDHM; PIG19

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Product Description:

LDHA mouse monoclonal antibody, clone OTI2D11 (formerly 2D11)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LDHA (RC209378, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LDHA. Positive lysates LY401712 (100ug) and LC401712 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LDHA monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500568)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500568)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500568)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500568)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500568)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LDHA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500568)
Immunoprecipitation (IP) of LDHA by usin
Immunoprecipitation (IP) of LDHA by using TrueMab monoclonal anti-LDHA antibodies (Negative control: IP without adding anti-LDHA antibody.). For each experiment, 500ul of DDK tagged LDHA overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LDHA antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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