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DHFR Mouse Monoclonal Antibody [Clone ID: OTI6G7]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

IF, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Monkey; Dog; Mouse; Rat

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full-length protein expressed in 293T cell transfected with human DHFR expression vector

Alternative Names:

DHFRP1; DYR

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Product Description:

Anti-DHFR (Dihydrofolate reductase) mouse monoclonal antibody, clone OTI6G7 (formerly 6G7)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DHFR (RC200089, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHFR. Positive lysates LY400271 (100ug) and LC400271 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DHFR monoclonal antibody.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-DHFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500543, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-DHFRmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500543, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-DHFRmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500543, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-DHFRmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500543, Dilution 1:50)
Anti-DHFR mouse monoclonal antibody (TA5
Anti-DHFR mouse monoclonal antibody (TA500543) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DHFR (RC200089).
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