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GBE1 Mouse Monoclonal Antibody [Clone ID: OTI4A1]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

FC, IF, IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Mouse; Rat

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell.

Alternative Names:

APBD; GBE; GSD4

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Product Description:

GBE1 mouse monoclonal antibody, clone OTI4A1 (formerly 4A1)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (RC204152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1. Positive lysates LY400056 (100ug) and LC400056 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GBE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500846)
Anti-GBE1 mouse monoclonal antibody (TA5
Anti-GBE1 mouse monoclonal antibody (TA500846) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1 (RC204152).
Immunofluorescent staining of HepG2 cell
Immunofluorescent staining of HepG2 cells using anti-GBE1 mouse monoclonal antibody (TA500846).
Immunoprecipitation (IP) of GBE1 by usin
Immunoprecipitation (IP) of GBE1 by using TrueMab monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Flow cytometric Analysis of Hela cells,
Flow cytometric Analysis of Hela cells, using anti-GBE1 antibody (TA500846), (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells
Flow cytometric Analysis of Jurkat cells, using anti-GBE1 antibody (TA500846), (Red), compared to a nonspecific negative control antibody, (Blue).
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