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GAD67 (GAD1) Mouse Monoclonal Antibody [Clone ID: OTI5D8]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
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Applications:

IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Mouse; Rat

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full-length protein expressed in 293T cell transfected with human GAD1 expression vector

Alternative Names:

CPSQ1; DEE89; GAD; SCP

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Product Description:

Anti-GAD1 (GAD67) mouse monoclonal antibody, clone OTI5D8 (formerly 5D8)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAD1 (RC207226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1. Positive lysates LY400290 (100ug) and LC400290 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug)
Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-GAD1 monoclonal antibody (1:200).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500330, Dilution 1:50)
Immunoprecipitation of GAD1 by using Tru
Immunoprecipitation of GAD1 by using TrueMab monoclonal anti-GAD1 antibodies (Negative control: IP without adding anti-GAD1 antibody.). For each experiment, 500ul of DDK tagged GAD1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GAD1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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