www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: OTI9C6]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£533.00 Shipping is calculated in checkout
Applications:

IF, IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.

Alternative Names:

CD32; CD32A; CDw32; FCG2; FcGR; FCGR2; FCGR2A1; IGFR2

image 0
image 1
image 2
Product Description:

FCGR2A (CD32) mouse monoclonal antibody, clone OTI9C6 (formerly 9C6)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (RC205786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A. Positive lysates LY402871 (100ug) and LC402871 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug)
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-FCGR2A monoclonal antibody at 1:200.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500645)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500645)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500645)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500645)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500645)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500645)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500645)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500645)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500645)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500645)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500645)
Anti-FCGR2A mouse monoclonal antibody (T
Anti-FCGR2A mouse monoclonal antibody (TA500645) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A (RC205786).
Immunoprecipitation (IP) of FCGR2A by us
Immunoprecipitation (IP) of FCGR2A by using TrueMab monoclonal anti-FCGR2A antibodies (Negative control: IP without adding anti-FCGR2A antibody.). For each experiment, 500ul of DDK tagged FCGR2A overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FCGR2A antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Details Cat number & supplier Size Price
Goat anti-Mouse IgG Heavy and Light Chain Antibody HRP Conjugated A90-116P · Bethyl Laboratories, Inc.
A90-116P
Bethyl Laboratories, Inc.
1 mg £91.20
1 mg
view
Goat anti-Mouse IgG Heavy and Light Chain Cross-Adsorbed Antibody HRP Conjugated A90-216P · Bethyl Laboratories, Inc.
A90-216P
Bethyl Laboratories, Inc.
0.5 mg £99.20
0.5 mg
view