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Protein Kinase A regulatory subunit I alpha (PRKAR1A) Mouse Monoclonal Antibody [Clone ID: OTI6C7]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
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Applications:

IF, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Mouse; Rat

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full-length protein expressed in 293T cell transfected with human PRKAR1A expression vector

Alternative Names:

ACRDYS1; ADOHR; CAR; CNC; CNC1; PKR1; PPNAD1; PRKAR1; TSE1

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Product Description:

Anti-PRKAR1A (PKA regulatory subunit I alpha) mouse monoclonal antibody, clone OTI6C7 (formerly 6C7)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR1A (RC212810, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1A. Positive lysates LY403945 (100ug) and LC403945 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKAR1A monoclonal antibody.
Western blot analysis of extracts (10ug)
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PRKAR1A monoclonal antibody (1:200).
Equivalent amounts of cell lysates (10 u
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and PRKAR1A-Knockout 293T cells (KO, Cat# LC811860) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1A monoclonal antibody TA500641, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PRKAR1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500641, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500641, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500641, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500641, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500641, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500641, Dilution 1:50)
Figure from citation: PRKAR1A IHC in a C
Figure from citation: PRKAR1A IHC in a CNC-associated cardiac myxoma. A, A photomicrograph of the specimen at low power exhibits the robust reactivity of the adjacent normal myocardium with antibodies directed against PRKAR1A. B, A high-power photomicrograph exhibits the lack of reactivity seen in the neoplastic (myxoma) cells, although the intratumoral histiocytes are still strongly reactive. Dilution: 1:8000 View Citation
Anti-PRKAR1A mouse monoclonal antibody (
Anti-PRKAR1A mouse monoclonal antibody (TA500641) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR1A (RC212810).
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