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Leukotriene A4 hydrolase (LTA4H) Mouse Monoclonal Antibody [Clone ID: OTI9C5]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
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Applications:

IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Mouse; Rat

Antibody Isotype:

IgG2b

Antibody Type:

Monoclonal

Immunogen:

Full-length protein expressed in 293T cell transfected with human LTA4H expression vector

Alternative Names:

leukotriene A4 hydrolase

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Product Description:

Anti-LTA4H (Leukotriene A4 hydrolase) mouse monoclonal antibody, clone OTI9C5 (formerly 9C5)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LTA4H (RC207617, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LTA4H. Positive lysates LY424467 (100ug) and LC424467 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug)
Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-LTA4H monoclonal antibody (1:200).
Equivalent amounts of cell lysates (10 u
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and LTA4H-Knockout 293T cells (KO, Cat# LC812368) were separated by SDS-PAGE and immunoblotted with anti-LTA4H monoclonal antibody TA500636, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-LTA4Hmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500636, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-LTA4Hmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500636, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-LTA4Hmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500636, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-LTA4Hmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500636, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-LTA4Hmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500636, Dilution 1:50)
Immunoprecipitation of LTA4H by using Tr
Immunoprecipitation of LTA4H by using TrueMab monoclonal anti-LTA4H antibody (Negative control: IP without adding anti-LTA4H antibody). For each experiment, 500ul of DDK tagged LTA4H overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LTA4H antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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