www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

loading...
loading...
  1. home
  2. OriGene
  3. TA500583
*

Alpha B Crystallin (CRYAB) Mouse Monoclonal Antibody [Clone ID: OTI6D11]

Catalogue number:
Add to favourites
TA500583
Supplier:
OriGene
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£533.00
add to basket
Shipping is calculated in checkout
Applications:

FC, IF, IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Rat; Monkey; Mouse

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.

Alternative Names:

CMD1II; CRYA2; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2

image 0
image 1
image 2
Product Description:

CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI6D11 (formerly 6D11)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (RC202718, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB. Positive lysates LY419682 (100ug) and LC419682 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CRYAB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot analysis of extracts (10ug)
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-CRYAB monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500583)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded human smooth muscle cancer tissue using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval, TA500583). Data courtesy of a collaborative pathologist.
Anti-CRYAB mouse monoclonal antibody (TA
Anti-CRYAB mouse monoclonal antibody (TA500583) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB (RC202718).
Immunofluorescent staining of HT29 cells
Immunofluorescent staining of HT29 cells using anti-CRYAB mouse monoclonal antibody (TA500583).
Immunoprecipitation (IP) of CRYAB by usi
Immunoprecipitation (IP) of CRYAB by using TrueMab monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC
HEK293T cells transfected with either RC202718 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody (TA500583), and then analyzed by flow cytometry.
Details Cat number & supplier Size Price
Goat anti-Mouse IgG Heavy and Light Chain Antibody HRP Conjugated A90-116P · Bethyl Laboratories, Inc.
A90-116P
Bethyl Laboratories, Inc.		 1 mg £114.00
1 mg
view
Goat anti-Mouse IgG Heavy and Light Chain Cross-Adsorbed Antibody HRP Conjugated A90-216P · Bethyl Laboratories, Inc.
A90-216P
Bethyl Laboratories, Inc.		 0.5 mg £124.00
0.5 mg
view