Alpha B Crystallin (CRYAB) Mouse Monoclonal Antibody [Clone ID: OTI3E3]

Name: Alpha B Crystallin (CRYAB) Mouse Monoclonal Antibody [Clone ID: OTI3E3]
Brand: OriGene
SKU: CBSKU_00118713
Price: 533 GBP
Availability: InStock

Catalogue number:

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TA500580

Supplier:

OriGene

Size:

100 µl _$$_

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£533.00

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Applications:

IF, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Mouse; Rat

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.

Alternative Names:

CMD1II; CRYA2; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2

Data sheet: View or download
MSDS: View or download

Product Description:

CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI3E3 (formerly 3E3)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (RC202718, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB. Positive lysates LY419682 (100ug) and LC419682 (20ug) can be purchased separately from OriGene.

Immunofluorescent staining of HT29 cells using anti-CRYAB mouse monoclonal antibody (TA500580).

Immunoprecipitation (IP) of CRYAB by using TrueMab monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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