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Glucose Transporter 5 GLUT5 (SLC2A5) Mouse Monoclonal Antibody [Clone ID: OTI12F2]

Catalogue number:
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TA500575
Supplier:
OriGene
Size:
100 µl _$$_
Product is available in:
  • UK
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£533.00
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Applications:

FC, IF, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full length human recombinant protein of human SLC2A5 (NP_003030) produced in HEK293T cell.

Alternative Names:

GLUT-5; GLUT5

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Product Description:

SLC2A5 (GLUT5) mouse monoclonal antibody, clone OTI12F2 (formerly 12F2)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC2A5 (RC200418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A5. Positive lysates LY401061 (100ug) and LC401061 (20ug) can be purchased separately from OriGene.
Immunofluorescent staining of HT29 cells
Immunofluorescent staining of HT29 cells using anti-SLC2A5 mouse monoclonal antibody (TA500575).
Immunoprecipitation (IP) of SLC2A5 by us
Immunoprecipitation (IP) of SLC2A5 by using TrueMab monoclonal anti-SLC2A5 antibodies (Negative control: IP without adding anti-SLC2A5 antibody.). For each experiment, 500ul of DDK tagged SLC2A5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC2A5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC
HEK293T cells transfected with either RC200418 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLC2A5 antibody (TA500575), and then analyzed by flow cytometry.
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