MagPureTM 96-well Plasmid Purification Kit

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BioVision's MagPureTM 96-well Plasmid Purification Kit combines the efficiency of magnetic bead technology with alkaline-SDS lysis of bacterial cells to purify high quality plasmid DNA. The kit is used to extract and purify plasmids using a 96-well Lysate Clearance Plate and magnetic particles. The cleared lysate obtained using the 96-well clearance plate in the first step is further purified using magnetic beads. The proprietary magnetic particles bind to DNA or RNA avidly, but reversibly under optimal conditions. The new 96-well Lysate Clearance Plate removes the need of time-consuming centrifugation to clear bacterial alkaline lysates. Proteins and other contaminants are removed and the nucleic acids can then be easily eluted with deionized water or low salt buffer. The purified plasmid can be used directly for automated fluorescent DNA sequencing and other standard molecular biology techniques such as restriction enzyme digestion. 1 ml of overnight culture in LB medium typically yields 10-15 µg high-copy plasmid DNA.