Carbohydrates are one of the most important components in many foods and the most abundant biomolecules present in all living organisms. Quantitation of total carbohydrate content is an essential analytical procedure in many fields including food and beverage products, agricultural products, medicinal products, vaccine pharmaceuticals etc. BioVision’s High Sensitivity Carbohydrate Assay Kit is a simple, sensitive, robust assay for the quantification of carbohydrates even when present in low concentration. The assay can be used for the quantitative determination of any carbohydrates that contain neutral sugars and uronic acids. The reaction is a modification of phenol-sulfuric acid method. In this assay, carbohydrates are hydrolysed and converted to hydroxyfurfural and furfural, which then react with a highly sensitive developer to form a colored complex, detected spectrophotometrically at 500 nm.
Biochemical and cellular assays