Epiandrosterone, or 3β-androsterone, also known as 3β-hydroxy-5a-androstan-17-one or 5α-androstan-3β-ol17-one, is a steroid hormone with weak androgenic activity. It is a natural metabolite of dehydroepiandrosterone (DHEA) via the 5α-reductase enzyme. BioVision’s Epiandrosterone ELISA kit is a competitive ELISA assay for the quantitative measurement of Epiandrosterone in urine, saliva, and fecal samples, or in extracted serum and plasma. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An Epiandrosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to Epiandrosterone to each well. After incubation the plate is washed and substrate is added. The substrate reacts with the bound Epiandrosterone -peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength.