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TIMP-3 (Human) ELISA Kit

Supplier:
Catalogue number:
E4842-100
Size:
96 assays
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£671.00 Estimated delivery Wed 23 Jun
Shipping is calculated in checkout

TIMP-3 belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localised to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. BioVision’s TIMP-3 (Human) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-3. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of TIMP-3 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human TIMP-3. The concentration of Human TIMP-3 in the samples can be calculated by comparing the OD of the samples to the standard curve.

  • Data sheet/protocol: View or download
  • MSDS: View or download
  • Applications: in-vitro quantitative determination of Human TIMP-3 concentrations in serum, plasma and other biological fluids.
Product Type:

ELISAs

Storage Temp:

4°C

UNSPSC Code:

41116126

TIMP-3 (Human) ELISA Kit

TIMP-3 (Human) ELISA Kit

Details Cat number & supplier Size Price
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