FITC-Tyramide
98%
526
1-(3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)-3-(4-hydroxyphenethyl)thiourea; Fluorescein isothiocyanate-tyramide
- Data sheet: View or download
- MSDS: View or download
Tyramide signal amplification is an enzyme-catalyzed deposition of tyramide on or near a protein or nucleic acid in situ. The enzyme horseradish peroxidase (HRP) converts the labelled tyramide substrate into a highly reactive form that can covalently bind to tyrosine residues on target molecules at or near the HRP, in the presence of low concentrations of hydrogen peroxide. Labelled FITC-tyramide reagent enhances both the fluorescent and colorimetric detection sensitivity. FITC-tyramide is used in multiplex immunohostochemistry (IHC) and Fluorescent in situ hybridization (FISH). FITC-tyramide is useful for detecting marine picoplankton, in situ imaging of proteins and studying the effects of reactive oxygen species on extracellular matrix proteins.
-20°C