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Comprehensive Epigenetic Profiling and Screening Services

Molecular Biology Services

Vector Shuttling & Clone Modification Service

Assemble DNA Constructs Simply & Efficiently Using The Gibson Assembly® Method

Methyltransferase & Demethylase Screening

Cambridge Bioscience offers access to the only Gold-Standard radioisotope-based service for both methyltransferase and demethylase assay profiling and low-cost high-throughput screening services.

Benefits of Using the Methyltransferase and Demethylase HotSpotSM Assay
• Only assay technology using nucleosome, histone protein and peptide or DNA as substrates
• Exclusive recombinant proteins validated for purity and activity in drug discovery applications
• Largest methyltransferase and demethylase panel in the industry
• Stringent control system - one standard control compound included in every assay
• No need for substrate labelling
• No antibody requirement
• Minimum false positives & negatives
• No intermediate dilution
• No interference from fluorescent compounds
• Small compound requirement - only nanolitres of compound needed

Methyltransferase & Demethylase Services Available
• Larger scale HTS
• Large scale single dose, duplicate profiling
• IC50 profiling — 10 dose with curve fitting
• Ki determination
• Custom assay development

Methyltransferase & Demethylase Screening Targets

Methyltransferase & Demethylase Assays
Methyltransferases
ASH1L MLL2 Complex PRMT6
COMT MLL3 Complex PRMT7
COMT (V108M) MLL4 Complex PRMT8
DNMT1 NSD1 SET1B
DNMT3a NSD2 SET7
DNMT3b NSD2 (E1099K) SET8
DNMT3b/DNMT3L NSD2 (T1150A) SETD2
Dot1L NSD3 SMYD1
EZH1 Complex PRDM9 SMYD2
EZH2 (Y641F) Complex PRMT1 SUV39H1
EZH2 Complex PRMT3 SUV39H2
G9a PRMT4 SUV420H1-tv2
GLP PRMT5 (non-complex)  
MLL1 Complex PRMT5/MEP50  
Demethylases
KDM4A KDM5A KDM6B
KDM4D KDM5B LSD1
 

Example Data
View example methyltransferase profiling and screening service data Here
View example demethylase profiling and screening service data Here

Contact Our Specialist
To discuss a methyltransferase or demethylase screening project with our Reaction Biology Specialist, please Click Here

Other Epigenetic Assays Available
HDAC/HAT, Bromodomain, DUB and PARP assays are also available. To find out more, please Click Here
Speak To Our Reaction Biology Specialist Vashu
Tonbo Biosciences