Methyltransferase & demethylase screening
Cambridge Bioscience offers access to the only Gold-Standard radioisotope-based service for both methyltransferase and demethylase assay profiling and low-cost high-throughput screening services.
Benefits of using the methyltransferase and demethylase HotSpotSM assay
• Only assay technology using nucleosome, histone protein and peptide or DNA as substrates
• Exclusive recombinant proteins validated for purity and activity in drug discovery applications
• Largest methyltransferase and demethylase panel in the industry
• Stringent control system - one standard control compound included in every assay
• No need for substrate labelling
• No antibody requirement
• Minimum false positives and negatives
• No intermediate dilution
• No interference from fluorescent compounds
• Small compound requirement - only nanolitres of compound needed
Methyltransferase and demethylase services available
• Larger scale HTS
• Large scale single dose, duplicate profiling
• IC50 profiling — 10 dose with curve fitting
• Ki determination
• Custom assay development
Methyltransferase and demethylase screening targets
| Methyltransferase and demethylase assays | ||
|---|---|---|
| Methyltransferases | ||
| ASH1L | MLL2 complex | PRMT6 |
| COMT | MLL3 complex | PRMT7 |
| COMT (V108M) | MLL4 complex | PRMT8 |
| DNMT1 | NSD1 | SET1B |
| DNMT3a | NSD2 | SET7 |
| DNMT3b | NSD2 (E1099K) | SET8 |
| DNMT3b/DNMT3L | NSD2 (T1150A) | SETD2 |
| Dot1L | NSD3 | SMYD1 |
| EZH1 Complex | PRDM9 | SMYD2 |
| EZH2 (Y641F) Complex | PRMT1 | SUV39H1 |
| EZH2 Complex | PRMT3 | SUV39H2 |
| G9a | PRMT4 | SUV420H1-tv2 |
| GLP | PRMT5 (noncomplex) | |
| MLL1 complex | PRMT5/MEP50 | |
| Demethylases | ||
| KDM4A | KDM5A | KDM6B |
| KDM4D | KDM5B | LSD1 |
Example data
View example methyltransferase profiling and screening service data.
View example demethylase profiling and screening service data.
Contact our specialist
Discuss a methyltransferase or demethylase screening project with our reaction biology specialist.
Other epigenetic assays available
HDAC/HAT, Bromodomain, DUB and PARP assays are also available.
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