Methyltransferase & Demethylase Screening
Cambridge Bioscience offers access to the only Gold-Standard radioisotope-based service for both methyltransferase and demethylase assay profiling and low-cost high-throughput screening services.
Benefits of Using the Methyltransferase and Demethylase HotSpotSM Assay
• Only assay technology using nucleosome, histone protein and peptide or DNA as substrates
• Exclusive recombinant proteins validated for purity and activity in drug discovery applications
• Largest methyltransferase and demethylase panel in the industry
• Stringent control system - one standard control compound included in every assay
• No need for substrate labelling
• No antibody requirement
• Minimum false positives & negatives
• No intermediate dilution
• No interference from fluorescent compounds
• Small compound requirement - only nanolitres of compound needed
Methyltransferase & Demethylase Services Available
• Larger scale HTS
• Large scale single dose, duplicate profiling
• IC50 profiling — 10 dose with curve fitting
• Ki determination
• Custom assay development
Methyltransferase & Demethylase Screening Targets
| Methyltransferase & Demethylase Assays | ||
|---|---|---|
| Methyltransferases | ||
| ASH1L | MLL2 Complex | PRMT6 |
| COMT | MLL3 Complex | PRMT7 |
| COMT (V108M) | MLL4 Complex | PRMT8 |
| DNMT1 | NSD1 | SET1B |
| DNMT3a | NSD2 | SET7 |
| DNMT3b | NSD2 (E1099K) | SET8 |
| DNMT3b/DNMT3L | NSD2 (T1150A) | SETD2 |
| Dot1L | NSD3 | SMYD1 |
| EZH1 Complex | PRDM9 | SMYD2 |
| EZH2 (Y641F) Complex | PRMT1 | SUV39H1 |
| EZH2 Complex | PRMT3 | SUV39H2 |
| G9a | PRMT4 | SUV420H1-tv2 |
| GLP | PRMT5 (non-complex) | |
| MLL1 Complex | PRMT5/MEP50 | |
| Demethylases | ||
| KDM4A | KDM5A | KDM6B |
| KDM4D | KDM5B | LSD1 |
Example Data
View example methyltransferase profiling and screening service data Here.
View example demethylase profiling and screening service data Here.
Contact Our Specialist
To discuss a methyltransferase or demethylase screening project with our Reaction Biology Specialist, please Click Here.
Other Epigenetic Assays Available
HDAC/HAT, Bromodomain, DUB and PARP assays are also available. To find out more, please Click Here.
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