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Product types:
Locus-Specific 5-mC Analysis - MethylCheck™
Custom Lentiviral Cloning Services
Comprehensive Epigenetic Profiling and Screening Services

Assemble DNA Constructs Simply & Efficiently Using The Gibson Assembly® Method

Histone deacetylase & acetylase screening

Cambridge Bioscience offers a fully defined, flexible profiling and screening service for potential drug inhibitors of HDACs/HATs. Using specific substrates in either radioisotope-based or fluorogenic-based functional assays, these highly sensitive and highly reproducible assays provide minimal false positives and negatives. These features make this service ideal for ensuring the right drug candidates are selected and progressed through the pipeline. Testing from regular profiling to miniaturised HTS platform is also available.

Benefits of using the HDAC and HAT screening and profiling service
• Gold standard radioisotope-based assay service for HATs or fluorogenic peptide substrate assay for HDACs
• Flexible - available for profiling and low cost high-throughput screening
• High sensitivity
• High reproducibility
• No antibody requirement
• Minimum false positives and negatives
• No intermediate dilution
• Small compound requirement - only nanolitres of compound required

HDAC and HAT services available
• Larger scale HTS
• Large scale, single dose, duplicate profiling
• IC50 profiling — 10 dose with curve fitting
• Ki determination
• Specific substrate for class2a HDACs used
• Custom assay development available

HDAC and HAT screening targets

HDAC and HAT assays
CBP HDAC8 Nuclear extract
HDAC1 HDAC9-Class2a sub p300
HDAC2 HDAC10 pCAF
HDAC3 HDAC11 SIRT1
HDAC4-Class2a sub hGCN5 SIRT2
HDAC5-Class2a sub KAT5 SIRT3
HDAC6 MYST2 SIRT5
HDAC7-Class2a sub MYST4  


Example data
View example data produced by the HDAC and HAT profiling and screening service.

Contact our specialist
Discuss a HDAC or HAT screening project with our reaction biology specialist.


Other epigenetic assays available
Methyltransferase, demethylase, bromodomain, DUB and PARP assays are also available.

Speak To Our Reaction Biology Specialist Vashu