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Frozen human cord blood buffy coat

Cambridge Bioscience offers access to frozen human cord blood buffy coat collected in the UK from fully traceable, HTA-licensed facilities. With 99 percent of red cells removed, this frozen cord blood buffy coat contains a concentrated supply of total nucleated cells (TNCs) including CD34+ cells.

TNC and CD34 count
The TNC and CD34+ counts are assessed using anti-CD45 and anti-CD34+ staining by FACS, together with a cell viability dye post-thaw on an aliquot of the cord blood unit. An expected true viable TNC or CD34+ post-thaw count will be provided with each sample.

Benefits of our frozen cord blood buffy coat service
• UK-based centres
• Human Tissue Authority (HTA) licensed collection and banking facilities
• Custom formats available
• Patented TotiCyte technology removes 99 percent of red cells
• Concentrated supply of TNCs - over 130 million to 7 billion cells
• Cell count and viability measurement provided with each sample
• Donors prescreened before collection and dispatch
• All processing carried out in facilities to GMP and ISO 9001 standards
• Samples consented for genetic analysis and commercial research purposes
• Collection and banking conforms and complies with the EU Cells and Tissues Directive

TotiCyte technology
Frozen cord blood buffy coat is isolated using novel, patented and CE marked reagent, TotiCyte, which retains 97 percent of haemopoietic stem cells post processing and at least 63 percent viability post-thaw. This results in 2.1 times more stem cells and three times more CD34+ cells recovered post-thaw than the current industry leading processing method.

Post Thaw Viability

Comparison of post-thaw viable CD34+ recovery from frozen cord blood buffy coats isolated using TotiCyte, industry leader and low-cost isolating reagents systems

Contact our team of blood specialists
To request a quote or find out more, please contact our blood specialists.

These products are for research use only and not for any human diagnostic or therapeutic use. It is your responsibility to ensure that you have necessary licensing and ethics approvals in place for the use, storage and disposal of any human tissue related material supplied by Cambridge Bioscience.

Contact Our Blood Specialists

Cells4Life - Fresh & Frozen Human Cord Blood Products