Tel: +44 (0)1223 316 855
life science research products, biological research products, biotechnology
close ...


Supplier:
Product types:

Molecular Biology Services

Custom miRNA qPCR Profiling
Find Your cDNA Simply & Easily With Our cDNA Search

Receive High Quality Leukaemia & Lymphoma Biospeciemens Within 24 Hours

 

CRISPR/Cas9 Services

Cambridge Bioscience offers access to efficient and effective CRISPR/Cas9 genome engineering services from System Biosciences’ experienced and successful team. Using the robust and reliable peer-reviewed CRISPR/Cas9 products, this service can save you time by covering all or part of the entire genome engineering workflow. 

Tier 1: Design & cloning of custom gRNA & HR donors
An important first step is the careful design and construction of guide RNAs (gRNAs) and homologous recombination (HR) donors. For gRNAs, SBI will design and clone a single gRNA or multiplex gRNAs against a target locus into any SBI SmartNuclease™ or SmartNickase™ vector (or customer provided gRNA cloning vector). For HR donors. SBI will design and clone homology arms into any SBI HR donor plasmid for knock-out, knock-in, tagging, or single nucleotide modification genome engineering projects. When a custom HR donor plasmid is ordered together with custom gRNA design and cloning, the donor vector will not contain full gRNA sequences in the homology arms to ensure full compatibility with gRNA to be used.

Tier 2: Full service custom cell line engineering
SBI will use custom Cas9 and HR donor constructs to engineer target cell lines for knock-out, knock-in, tagging, or single nucleotide modification applications. Use of HR donor is usually required and is typically ordered as a package with two gRNA constructs. SBI can screen resistant cells to identify a clonal line with the desired modification or deliver a mixed population of resistant cells for further characterisation

Successful Genome Engineering With SBI

Successful genome engineering with SBI—effective, efficient knockout of miR-21 in HEK293 cells. gRNA, HR Donor design (with Puro and GFP selection markers), implementation, and analysis performed by SBI’s genome engineering services team. (A) Low relative levels of miR-21—as measured by qPCR in GFP-positive clones—demonstrate the effectiveness of the approach. (B) After excision with Cre recombinase, the inserted GFP and Puro markers are efficiently excised, leaving only a single LoxP site from the HR Donor. From Ho, TT, et al. Targeting noncoding RNAs with the CRISPR/Cas9 system in human cell lines. Nucleic Acids Res. 2015 Feb 18; 43(3):e17. PMCID: PMC4330338.

Benefits Of Using These CRISPR/Cas9 Services
• Highly experienced scientist team
• Save time
• gRNA and/or HR donor design & construction
• Turn-around time as little as 1–2 weeks for gRNA cloning
• Turn-around time as little as 3–4 weeks for HR donor cloning

How To Order These Services
1. Contact our System Biosciences Specialist Here to discuss your requirements & receive a quote
2. Send us any necessary materials e.g. plasmids (optional)
3. Receive your constructs in 1–2 weeks for gRNA plasmids & 3–4 weeks for HR donor plasmids

Material Available For Download
SBI Brochure CRISPR Cas9 Services

Contact our SBI Specialist, Katie
Tonbo Biosciences