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Automated Cell Counter - EVE

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Automated Cell Imaging System - JuLI™ Stage

Cambridge Bioscience offers a fully automated, CO2 incubator-compatible system for digital multi-colour cell imaging. Recording cell behaviour in real-time or time lapse via a user friendly interface, the JuLI Stage creates high resolution images and movies for publication or presentation simply and quickly.

Benefits of Using JuLI™ Stage
• High resolution images & movies
• Real-time visual & graphical output
• Multi-channel imaging (GFP/RFP/DAPI & brightfield)
• User-friendly software
• User changeable objective lenses (4x, 10x & 20x)
• Fits in CO2 incubators
• Works with standard tissue culture consumables

Great Discounts On Ex-Demo JuLI Stages
We are offering great discounts on our ex-demo JuLI Stages. Full one year warranty included as standard. Stock is strictly limited. Request a quote Here

JuLI™ Stage Applications
1. Wound Healing
2. Time-lapse Images & Movies
3. Growth Curves
4. Fluidigm® Applications
5. Image Stitching
6. 3D Spheroid Culture
7. Fluorescence Expression
8. Apoptosis
9. Bacterial Infection of PBMCs

1. Wound Healing
Multiple positions across a wound can be visualised, the recovering surface can be quantitatively analysed and wound healing time-course experiments can be performed producing exceptional results in real time.

U2OS cells stably transfected with GFP (left) and RFP (right), seeded with a barrier plug between them. After removal of the plug, the cells are free to migrate across the gap.

2. Time-lapse Images & Movies
Multiple images across different time intervals can be captured and multicolour images and movies generated using single or multiple acquisition channels.
PA-317, SHSY-5, U2-OS, MCG-7 cells cultured for 40 hours after scratching.

3. Growth Curves
Growth curves, monolayer confluence and cell morphology can be studied over extended time periods with graphs, images and tabulated data easily exported.

4. Fluidigm® Applications
High-quality results can be achieved with with Fluidigm® integrated fluidic circuits (IFC). Cells can be imaged automatically, cell viability can be recorded and cell counts can be performed with high sensitivity and accuracy in each chamber of a Fluidigm® C1 IFC chip. Fluidigm is a registered trademark of Fluidigm Corporation.

5. Image Stitching
Multiple tiled scans of an entire well can be taken and then brought together to create a single stitched image. Tissue sections, stem cell colonies or multiple cells can be viewed in each well.

6. 3D Spheroid Culture

Integrated z-stacking enables focussed images of multiple areas of 3D cultures with a couple of clicks. Spheroids and other cell formations can be monitored without worrying about focus.
U2OS green and red mono-stained cell culture in round bottomed plate over 72 hours. The cells initially form a dense spheroid. After treatment with cisplatin the spheroid begins to disintegrate, but the core is undamaged throughout.

7. Fluorescence Expression
Cell confluence can be measured using brightfield and fluorescence channels (GFP & RFP). Transfection efficiencies and cell survival can be simultaenously studied in each field of view.
40 hour culture of PA317 cells transfected with an RFP construct. Expression becomes apparent after roughly 5 hours, increasing throughout.

8. Apoptosis
With fluorescence used in most commercially available apoptosis kits detected, create high resolution fluorescent & brightfield images and videos of apoptosis in multiple wells.

Effect of Camptothecin (16 uM) with intervals of 20 minutes in U-2 OS. Time-lapse JuLI Stage images taken in the presence of Caspase-3/7 reagent (4 uM).

9. Bacterial Infection of PBMCs

Capturing image of PBMC  infection exp. with GFP transformed bacteria. JuLI Stage movie by time-lapse function.

Technical Specifications

JuLI™ Stage Citation List

JuLI™ Stage Demonstration
Request a free demonstration, technical presentation or find out more by contacting our JuLI™ Stage specialists Here
Contact Our NanoEnTek Specialist, Vashu