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3D cell culture incubator

The CERO 3D incubator and bioreactor from OMNI Life Science (OLS) utilises unique 3D cell culture technology to boost your stem cell, spheroid, organoid and tissue research. Offering improved maturation with no emedding substrate required, the CERO 3D significantly reduces apoptoses and necrosis resulting in the highest levels of viability and homogeneity. 

How does the CERO 3D work?
The CERO 3D creates the optimal cell culture environment by monitoring and controlling temperature, pH and CO2 levels. Contatining 1 to 4 individually controlled CEROtubes, each with a volume up to 50 ml, provide the highest biomass yields in a standardised and reproducible conditions, with minimal handling requirements. CEROtubes feature small fins and a flat bottom to enable gentle cultivation conditions with reduced shear stress on your cells.

Benefits of using the CERO 3D
• Temperature, pH annd CO2 levels monitored and controlled
• Improved viability and maturation
• Standardised and reproducible conditions maintained
• No emberring substrate required
• Significantly reduce apoptosis and necrosis
• No shear force stress inflicted on cells
• Suitable for long-term cultivation for >1 year

CERO 3D applications

  • Pluripotent stem cells

The CERO provides a solution for scale-up and automation platforms, simplification and cost reduction of stem cell expansion projects, cell-based drug discovery, toxicity testing and regenerative medicine.

Human iPSC after expansion in CERO 3D Incubator & Bioreactor tested for pluripotency.

Human iPSC after expansion in CERO 3D Incubator & Bioreactor tested for differentiation in three germ layers.

Left: Human iPSC after expansion in CERO 3D Incubator & Bioreactor tested for pluripotency. Right: Human iPSC after expansion in CERO 3D Incubator & Bioreactor tested for differentiation in three germ layers.

  • Hepatitis virus research model - spheroids

Long-term 3D dimensional cell culture holds great promise for disease modeling and drug discover, however many scientists struggle with technical limitations when working with spheroids in long-term culture. The CERO provides revolutionary technology to enable scientists to perform experiments they were not able to do before.

Cells are positive for cell proliferation marker KI67

Cells are negative for apoptosis marker Casp.cl.3

Cells are positive for albumin

Left: Cells are positive for cell proliferation marker KI67. Centre: Cells are negative for apoptosis marker Casp.cl.3. Right: Cells are positive for albumin.

  • Organoids as tumour tissue model

The CERO offers an efficient and standardised method to generate and maintain high yields of homogeneous organoids for use as tools in cancer research.

Gastric organoids (bright field) at day 7 and after splitting on day 22 expanding from small cysts that expand to bigger spheres

HE staining of Gastric organoids (2a) showing single layer of epithelia cells composed by the different cell types found in the stomach as seen in the PCR results (2b): expression of gastric mucins MUC5AC and MUC6, trefoil factors and pepsinogen, for instance. There are also stem cells markers like Lgr5 and Sox2.

Left: Gastric organoids (bright field) at day 7 and after splitting on day 22 expanding from small cysts that expand to bigger spheres. Right: HE staining of Gastric organoids (2a) showing single layer of epithelia cells composed by the different cell types found in the stomach as seen in the PCR results (2b): expression of gastric mucins MUC5AC and MUC6, trefoil factors and pepsinogen, for instance. There are also stem cells markers like Lgr5 and Sox2.

Material available for download
CERO 3D brochure

CERO 3D incubator and bioreactor