ZymoTaq DNA Polymerase from Zymo Research is a heat-activated, “hot start” polymerase with 3′-terminal transferase activity. Specifically designed for the amplification of bisulphite-treated DNA for methylation detection, the addition of “A” overhangs to amplified DNA also makes it ideal for use in TA-cloning or conventional PCR.
Benefits of using ZymoTaq DNA Polymerase
• Heat-activated and thermostable for reduced primer dimer and non-specific product formation
• Specific amplicons generated with little or no by-product formation
• Simple and easy to use
Efficient PCR amplification of bisulphite-treated DNA for methylation detection. Figure shows a 274 bp product amplified from bisulphited-treated DNA using ZymoTaq DNA polymerase vs polymerases from supplier Y and N. In each case, equal amounts of bisulphite-treated DNA (EZ DNA Methylation-Lightning™ kit from Zymo Research) were used for each duplicate PCR reaction and the products were visualised using the Agilent 2200 TapeStation® system.
Material available for download
ZymoTaq DNA Polymerase manual
TapeStation® is a registered trademark of Agilent Technologies, Inc.