Cambridge Bioscience offers a highly effective buffer system for suppressing background signal caused by non-specific protein binding and charged fluorescent dyes for visible and near-IR fluorescent western blotting (WB). Eliminating non-specific bands across the entire membrane better than BSA, gelatin, or casein and using non-mammalian blocking agents for broad secondary antibody compatibility, the TrueBlack® WB blocking buffer kit is ideal for achieving optimal specificity and sensitivity for fluorescence-based WB.
Benefits of Using TrueBlack WB blocking buffer kit
• High background fluorescence blocked
• Non-specific bands eliminated
• PVDF and nitrocellulose membrane compatible
• For visible and near-IR fluorescent WBs
• TBS based buffer suitable for phosphoprotein detection
• Non-mammalian blocking agents
• Broad secondary antibody compatibility
• Trial size available
TrueBlack WB Blocking Buffer gives lower background fluorescence and better specificity compared to other buffers
Western detection of phospho-Erk1/2 in PDGF-stimulated NIH-3T3 cell lysate. Membranes were blocked with fish gelatin blocking buffer, LI-COR® Odyssey® TBS Blocking Buffer, or TrueBlack WB Blocking Buffer. Phosphorylated Erk was detected using rabbit anti-pErk1/2 and CF®680R donkey anti-rabbit antibodies.
Western detection of tubulin in HeLa cell lysate with mouse anti-tubulin and Alexa Fluor® 790 goat anti-mouse antibodies. Membranes blocked with fish gelatin blocking buffer or TrueBlack WB Blocking Buffer. The highly negatively charged Alexa Fluor® 790 labelled antibody showed non-specific binding to the PVDF membrane and cellular proteins, which was blocked by TrueBlack WB Blocking Buffer. Lanes 1-3: 10 ug, 1 ug, or 0.1 ug HeLa cell total protein.
• Fluorescence-based western blotting
Alexa Fluor is a registered trademark of Thermo Fisher Scientific.
LI-COR and Odyssey are registered trademarks of LI-COR Inc.
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