Benefits of using the TrueBlack™ Lipofuscin Autofluorescence Quencher
• Simple protocol
• Lipofuscin autofluorescence eliminated effectively
• High background fluorescence avoided
• Fluorescence imaging of human and aged animal tissues enabled
• Fluorescent antibodies and nuclear counterstains compatible
Note: TrueBlack™ is designed to quench autofluorescence caused by lipofuscin, it is not effective at reducing autofluorescence from other sources such as glutaraldehyde-induced fluorescence or endogenous fluorescence of collagen or elastin.
Lipofuscin autofluorescence in methanol-fixed adult human tissue sections. Untreated tissue (top row), lipofuscin appeared as fluorescent granules that fluoresced in all fluorescence channels. Sudan Black B (middle row) masked lipofuscin autofluorescence, but introduced background in the red and far-red channels. TrueBlack (bottom row) masked lipofuscin with minimal increase in fluorescence background (bottom row).
Material available for download
TrueBlack™ citation list
Note: product availability depends on country - see product detail page.
|Details||Cat number & supplier||Size||Price|
|TrueBlack® Lipofuscin Autofluorescence Quencher, 20X in DMF BT23007 · Biotium||