Cambridge Bioscience offers a highly effective buffer system for suppressing fluorescent background from both non-specific antibody binding and charged fluorescent dyes in cells and tissue sections after IF staining. Using non-mammalian blocking agents for broad secondary antibody compatibility, the TrueBlack® IF background suppressor system is an ideal solution highly efficient background blocking.
Benefits of using TrueBlack IF background suppressor system
• Non-specific background suppressed
• Cells or tissue sections IF stained
• Block and permeabilise in 10 minutes
• Non-mammalian blocking agents
• Broad secondary antibody compatibility
• Trial size available
TrueBlack IF Background Suppressor reduces non-specific binding of antibodies conjugated to charged fluorescent dyes
Methanol-fixed HeLa cells were blocked for 10 minutes with fish gelatin blocking buffer or TrueBlack® IF Background Suppressor, then stained with no primary antibody or mouse anti-tubulin antibody, followed by goat anti-mouse secondary antibody conjugated to the indicated dyes. Antibodies were diluted in the same buffer used for blocking.
For immunofluorescence blocking of cells or tissue sections
Rat intestine cryosection blocked with TrueBlack® IF Background Suppressor (Permeabilising) and stained with CF®555 Mix-n-Stain™ labelled anti-ZO1 (tight junctions, red) and NucSpot® 470 nuclear stain (nuclei, green), diluted in TrueBlack® IF Blocking Buffer (Permeabilising) for staining.
• Immunofluorescence staining
Materials Available To Download
TrueBlack® IF Background Suppressor System Protocol
Fluorescence Background Blockers Brochure
Note: product availability depends on country - see product detail page.
|Details||Cat number & supplier||Size||Price|
|TrueBlack® IF Background Suppressor System (Permeabilizing) BT23012-T · Biotium||
|TrueBlack® IF Background Suppressor System (Permeabilizing) BT23012 · Biotium||