The ToxOut™ Rapid Endotoxin Removal Kit is a convenient, refillable column-based kit for the simple, effective and rapid removal of endotoxin. With around 90 percent sample recovery and only five minutes per removal cycle, the ToxOut™ rapid endotoxin removal kit enables removal of endotoxin to the level of less than 0.05 EU/ml in solution via immobilised polymyxin B. Each column can be used up to five times and has a binding capacity of up to 9 x 108 EU/ml resin.
Benefits of using the ToxOut™ Rapid Endotoxin Removal Kit
• Five minute removal cycle
• Endotoxin removal down to less than 0.05 EU/ml
• Aroud 90 percent sample recovery
• Up to 9 x 108 EU/ml resin binding capacity
• Each column can be used up to five times
ToxOut™ Rapid Endotoxin Removal Kit removes more than 90 percent of endotoxin from protein solutions
Endotoxin capacities and endotoxin efficiencies were determined by challenging 0.1 ml resin with 1 x 108 EU/ml LPS in 200 μl BSA (10 mg/ml) solution. By reloading samples to the repeatedly regenerated column, the endotoxin spike is reduced to less than 0.05 EU/ml in the BSA solution. The highest endotoxin binding capacity is 9.99 x 108 EU/ml from the first cycle of detox. The average detox efficiency of five cycles of detox is 94.5 percent and the average protein recovery of five cycles of detox is 89.6 percent.
Material available for download
Note: product availability depends on country - see product detail page.