Total protein prestain kits enable easily labelling of proteins or cell lysates before electrophoresis. Providing highly linear quantitation on SDS-PAGE gels and western blot membranes, the total protein prestain kits are very easy to use.
Benefits of using the Total Protein Prestain Kits - Mix-n-Stain™
• Superior linearity for Western blot normalisation compared to housekeeping proteins
• Highly sensitive (detect as little as 1 ng protein)
• Fast and simple procedure
• Easily label proteins or cell lysates with near-IR CF® dyes before SDS-PAGE
• No purification needed after labelling
• Does not affect protein detection by antibodies
Mix-n-Stain™ Total Protein Prestain Kits for western blot normalisation
HeLa cell lysates in serial dilution are labelled with (A) the CF®770T total protein prestain kit or (B) mouse antitubulin primary antibody and CF®770T goat anti-mouse secondary antibody, both on PVDF membranes. Total protein content in cell lysate was determined by the bicinchoninic acid assay (BCA). (C) Plots of band intensity vs protein content for CF®770T labelled lysate (shown in A), CF®680T labelled lysate (membrane not shown), and tubulin WB (shown in B). The Mix-n-Stain™ Total Protein Prestain Kits show better linearity compared to the antibody labelling of housekeeping proteins.
In-gel fluorescence image of bovine serum albumin (BSA) labelled with the CF®680T Total Protein Prestaining Kit on SDS-PAGE gel
In-gel fluorescence image of bovine serum albumin (BSA) labelled with the CF®680T Total Protein Prestaining Kit on SDS-PAGE gel. Protein content for each lane ranges from 10 µg to 1 ng, from left to right. The bands above and below the major bands are from impurity proteins in the BSA sample. The excess dye runs to the very bottom of the gel. Insert: part of the image with enhanced brightness to visualise the bands with 10, 5, and 1 ng of BSA.