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Total Cholesterol & Cholesteryl Ester Assay Kit

This sensitive assay from BioVision enables the simple quantification of free cholesterol, cholesteryl esters (or both) by colourimetric or fluorometric methods. The Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit detects total cholesterol (cholesterol and cholesteryl esters) in the presence of cholesterol esterase or free cholesterol in the absence of cholesterol esterase. The assay is compatible with cell and tissue culture supernatants, urine, plasma and serum.

Assay process
The majority of the cholesterol in blood is in the form of cholesteryl esters which can be hydrolysed to cholesterol by cholesterol esterase under appropriate conditions. Cholesterol is then oxidized by cholesterol oxidase to yield H₂O₂. H₂O₂ reacts with a sensitive cholesterol probe to produce either colour (λmax = 570 nm) or fluorescence (Ex/Em = 535/587 nm). In the presence of cholesterol esterase, this assay detects total cholesterol (cholesterol and cholesteryl esters) and in the absence of cholesterol esterase, this assay detects free cholesterol. The amount of cholesteryl ester present can be determined by subtracting the value of free cholesterol from the total.

Benefits of using this Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit
• 60 minute simple procedure
• Quantification of free cholesterol, cholesteryl esters, or both
• Fluorometric or colourimetic detection
• Wide mammalian sample compatibility




Material available for download
Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit protocol

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit K603-100 · BioVision
K603-100
BioVision
100 assays £588.00
100 assays
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