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3D cell culture ECM hydrogels: TissueSpec

TissueSpec® ECM hydrogels from Xylyx provides cells with a tissue-specific, 3D cell culture substrate containing the full milieu of proteins and growth factors present in the native tissue for more physiologically relevant results.  With consistent lot-to-lot composition, cells can be cultured on the surface of or within the easy-to-use hydrogels.

Benefits of using TissueSpec ECM hydrogels
• Derived from normal porcine tissue
• Contains tissue-specific ECM components
• Supports cell & organoid cultures
• Compatible with standard cell culture protocols
• Lot-to-lot consistency
• Easy-to-use

TissueSpec ECM hydrogels for predictive 3D models of human cancer
Download the poster

TissueSpec ECM hydrogel use
TissueSpec ECM Hydrogel can be applied as a thin gel on which to culture cells and study cell activity, function as well as migration and invasion assays.To study cell-cell interactions and microtissue structures, encapsulate and culture cells or organoids within 3D TissueSpec ECM Hydrogel.

tissuespec_ecm_hydrogels

Hepatocyte function In TissueSpec liver ECM hydrogel

Low-density lipoprotein uptake comparison

Hepatocyte function in TissueSpec® Liver ECM Hydrogel

Hepatocyte function in TissueSpec® Liver ECM Hydrogel - fibrinogen secretion

Primary human hepatocytes cultured in thin hydrogels (1 mm, 4 mg/mL) of TissueSpec Liver matrix, Matrigel, and collagen I showed significantly higher (a) expression of LDL receptor and (b) secretion of fibrinogen (p<0.05) in TissueSpec Liver ECM Hydrogel compared to hepatocytes cultured in Matrigel and collagen I.

Maintenance of lung cell identity in TissueSpec lung ECM hydrogel

(a) Epithelial cell gene expression

Maintenance of lung cell identity in TissueSpec® Lung ECM Hydrogel - (a) Epithelial cell gene expression

(b) Epithelial cell marker expression

TissueSpec® Lung ECM Hydrogel Epithelial cell marker expression

(c) Airway progenitor cell maintenance

TissueSpec® Lung ECM Hydrogel - Airway progenitor cell maintenance - (c) Airway progenitor cell maintenance

(d) Basal cell enrichment

TissueSpec® Lung ECM Hydrogel - (d) Basal cell enrichment

Primary normal human bronchial epithelial (NHBE) cells were cultured on thin layers of TissueSpec Lung ECM Hydrogel or Matrigel for 10 days. TissueSpec Lung ECM Hydrogel supported (a,b) robust expression of normal lung epithelial cell markersand (c,d) significantly larger subpopulation of p63+ basal airway cells (p<0.05) compared to Matrigel.

Applications
• Cell culture
• Cancer research
• Stem cell research
• Tissue engineering
• Clinical applications
• Drug screening
• Organ-on-a-chip

Material available for download
TissueSpec ECM Hydrogel instructions for use 
TissueSpec ECM Hydrogels dissociation protocol
issueSpec ECM Hydrogels immunostaining protocol
TissueSpec Intestine ECM Hydrogel data
TissueSpec Bone ECM Hydrogel datasheet
TissueSpec Liver ECM Hydrogel datasheet
TissueSpec Lung ECM Hydrogel datasheet

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
TissueSpec® Bone ECM Hydrogel MTSBN101 · Xylyx
MTSBN101
Xylyx
1 ml £266.00
1 ml
view
TissueSpec® Heart ECM Hydrogel MTSHT101 · Xylyx
MTSHT101
Xylyx
1 ml £266.00
1 ml
view
TissueSpec® Intestine ECM Hydrogel MTSIN101 · Xylyx
MTSIN101
Xylyx
1 ml £266.00
1 ml
view
TissueSpec® Kidney ECM Hydrogel MTSKY101 · Xylyx
MTSKY101
Xylyx
1 ml £266.00
1 ml
view
TissueSpec® Liver ECM Hydrogel MTSLV101 · Xylyx
MTSLV101
Xylyx
1 ml £266.00
1 ml
view
TissueSpec® Lung ECM Hydrogel MTSLG101 · Xylyx
MTSLG101
Xylyx
1 ml £266.00
1 ml
view
TissueSpec® Skin ECM Hydrogel MTSSK101 · Xylyx
MTSSK101
Xylyx
1 ml £266.00
1 ml
view