3D cell culture ECM hydrogels: TissueSpec

TissueSpec® ECM hydrogels from Xylyx provides cells with a tissue-specific, 3D cell culture substrate containing the full milieu of proteins and growth factors present in the native tissue for more physiologically relevant results.  With consistent lot-to-lot composition, cells can be cultured on the surface of or within the easy-to-use hydrogels.

Benefits of using TissueSpec ECM hydrogels
• Derived from normal porcine tissue
• Contains tissue-specific ECM components
• Supports cell & organoid cultures
• Compatible with standard cell culture protocols
• Lot-to-lot consistency
• Easy-to-use

TissueSpec ECM hydrogels for predictive 3D models of human cancer
Download the poster

TissueSpec ECM hydrogel use
TissueSpec ECM Hydrogel can be applied as a thin gel on which to culture cells and study cell activity, function as well as migration and invasion assays.To study cell-cell interactions and microtissue structures, encapsulate and culture cells or organoids within 3D TissueSpec ECM Hydrogel.

Hepatocyte function In TissueSpec liver ECM hydrogel

Primary human hepatocytes cultured in thin hydrogels (1 mm, 4 mg/mL) of TissueSpec Liver matrix, Matrigel, and collagen I showed significantly higher (a) expression of LDL receptor and (b) secretion of fibrinogen (p<0.05) in TissueSpec Liver ECM Hydrogel compared to hepatocytes cultured in Matrigel and collagen I.

Maintenance of lung cell identity in TissueSpec lung ECM hydrogel

(a) Epithelial cell gene expression

(b) Epithelial cell marker expression

(c) Airway progenitor cell maintenance

(d) Basal cell enrichment

Primary normal human bronchial epithelial (NHBE) cells were cultured on thin layers of TissueSpec Lung ECM Hydrogel or Matrigel for 10 days. TissueSpec Lung ECM Hydrogel supported (a,b) robust expression of normal lung epithelial cell markersand (c,d) significantly larger subpopulation of p63+ basal airway cells (p<0.05) compared to Matrigel.

Applications
• Cell culture
• Cancer research
• Stem cell research
• Tissue engineering
• Clinical applications
• Drug screening
• Organ-on-a-chip

Testimonial
As a cancer cell biologist, it is always challenging to develop ideal cancer cell culture condition in 3D model to study cell-signaling pathways. Xylyx Bio offers bone and liver derived hydrogels, the two major sites for the prostate cancer metastasis. Those TissueSpec ECM Hydrogels are a perfect solution to study the effect of metastatic microenvironment on prostate cancer cell signaling.
Goutam Chakraborty, Ph.D, MSKCC

Custom TissueSpec ECM hydrogels
Standard TissueSpec ECM Hydrogels are derived from porcine tissues. However, the Xylyx platform technology allows matrix isolation from different tissue sources, including human tissues, according to specific requests. Discuss a custom project with our Xylyx specialist.

Material available for download
TissueSpec ECM Hydrogel instructions for use 
TissueSpec ECM Hydrogels dissociation protocol
issueSpec ECM Hydrogels immunostaining protocol
TissueSpec Intestine ECM Hydrogel data

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