www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

Synthetic long-read DNA library preparation: TELL-Seq

The Transposase Enzyme Linked Long-read Sequencing (TELL-Seq™) technology from Universal Sequencing Technology enables the preparation of sequencing-ready DNA libraries for synthetic long-read output. Providing a rapid and highly accurate solution for de novo sequencing and analysis of structural variations within small & large genomes (1MB – 5GB), the TELL-Seq kits add phasing capabilities to Illumina® instruments. This allows for the efficient sequencing of novel genomes by linking short-reads by using clonal barcoding technology, producing synthetic long-reads. 

Benefits of using the TELL-Seq library prep kits
• Enables simple and scalable genome analysis
• Generate between 4 (large genomes) to 12 (small genomes) Illumina®-compatible library preparations per kit
• Suitable for the sequencing of bacterial, fungal, plant and animal genomes
• Easy protocol: single tube process with <1 hour hands-on time
• Robust and reproducible data
• Low DNA input between 0.5ng to 5ng.
• Generate sequencing-ready library in just 3 hours
• Highly accurate for suboptimal DNA input samples or samples containing with high GC content

How TELL-Seq technology works

How it works

Applications
• Genome wide variant calling
• Structural variation detection
• Metagenomic studies
De novo sequencing assembly

Material available for download
User guide: TELL-Seq mixed sample demultiplexing
User guide: TELL-Seq human exome capture
User guide: TELL-Seq WGS Library Prep
Application note: targeted phasing of Cas9-excised human DNA
Application note: TELL-Seq De Novo assembly
TELL-Seq recommended packages

TELL-Seq citations

For more information or to discuss your project, please contact our NGS specialists.

Illumina is a registered trademark of Illumina, Inc.

How to assemble your Tell-Seq package

The Tell-Seq standard package is recommended for new users.

Step 1 - Select your enzyme mix
TELL-Seq™ WGS Library Reagent Box 1 Catalogue number: 100001
Step 2 - Select the Tell-Beads™
TELL-Seq™ WGS Library Reagent Box 2 Catalogue number: 100002
Step 3 - Add multiplex primers
TELL-Seq™ Library Multiplex Primer (1-8) Kit Catalogue number: 100003
Step 4 - Add Illumina® primers
TELL-Seq™ Illumina® Sequencing Primer Kit Catalogue number: 100004


Each component must be purchased separately.

The Tell-Seq high-throughput package is recommended for new users.

Step 1 - Select your enzyme mix
TELL-Seq™ WGS Library Reagent Box 1, HT24 Catalogue number: 100011
Step 2 - Select the Tell-Beads™
TELL-Seq™ WGS Library Reagent Box 2, HT24 Catalogue number: 100012
Step 3 - Add multiplex primers
TELL-Seq™ Library Multiplex Primer (1-8) Kit Catalogue number: 100003
Step 4 - Add Illumina® primers
TELL-Seq™ Illumina® Sequencing Primer Kit, HT Catalogue number: 100013
Optional step - add further multiplex primers, depending on your multiplex needs
TELL-Seq™ Library Multiplex Primer (9-16) Kit Catalogue number: 100009
TELL-Seq™ Library Multiplex Primer (17-24) Kit Catalogue number: 100010


Package is dependent on sequencer and project. Each component must be purchased separately.

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
TELL-Seq™ WGS Library Reagent Box 1 100001 · Universal Sequencing Technology
100001
Universal Sequencing Technology
1 kit £315.00
1 kit
view
TELL-Seq™ WGS Library Reagent Box 2 100002 · Universal Sequencing Technology
100002
Universal Sequencing Technology
1 kit £441.00
1 kit
view
TELL-Seq™ Library Multiplex Primer (1-8) Kit 100003 · Universal Sequencing Technology
100003
Universal Sequencing Technology
1 kit £132.00
1 kit
view
TELL-Seq™ Illumina Sequencing Primer Kit 100004 · Universal Sequencing Technology
100004
Universal Sequencing Technology
1 kit £58.00
1 kit
view
TELL-Seq™ WGS Library Reagent Box 1, HT24 100011 · Universal Sequencing Technology
100011
Universal Sequencing Technology
1 kit £1658.00
1 kit
view
TELL-Seq™ WGS Library Reagent Box 2, HT24 100012 · Universal Sequencing Technology
100012
Universal Sequencing Technology
1 kit £2371.00
1 kit
view
TELL-Seq™ Illumina Sequencing Primer Kit, HT 100013 · Universal Sequencing Technology
100013
Universal Sequencing Technology
1 kit £289.00
1 kit
view
TELL-Seq™ Library Multiplex Primer (17-24) Kit 100010 · Universal Sequencing Technology
100010
Universal Sequencing Technology
1 kit £132.00
1 kit
view
TELL-Seq™ Library Multiplex Primer (9-16) Kit 100009 · Universal Sequencing Technology
100009
Universal Sequencing Technology
1 kit £132.00
1 kit
view
TELL-Seq™ WGS Library Reagent Box 1, HT96 100015 · Universal Sequencing Technology
100015
Universal Sequencing Technology
1 kit £5896.00
1 kit
view
TELL-Seq™ WGS Library Reagent Box 2, HT96 100016 · Universal Sequencing Technology
100016
Universal Sequencing Technology
1 kit £8707.00
1 kit
view
TELL-Seq™ Target Blocker Kit 100019 · Universal Sequencing Technology
100019
Universal Sequencing Technology
8 reactions £95.00
8 reactions
view