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Swift 2S Turbo DNA Library Prep Kits

NGS library prep from limited samples
Library normalisation technology for NGS

Optimise Your Microbiomics Workflows with ZymoBIOMICS™ Microbial Community Standards

Library normalisation technology for NGS

Library normalisation technology for NGS

The Swift Normalase kit is a revolutionary library normalisation technology for NGS. Developed to save time and increase throughput by providing uniform sample processing with fewer handling steps, Swift Normalase is compatible with diverse library preparation methods and sample types to produce more evenly balanced sequence data.

Advantages of Normalase over traditional library quantification methods
• Normalisation is dictated by molarity rather than mass – ensures sequencer compatibility
• Quantification-free
• No need for individual concentration adjustment/fragment size estimates of each library
• Highly reproducible normalisation & robust performance across variable insert sizes

The Swift Normalase kit offers an efficient, robust, scalable & cost-effective enzymatic-based library normalisation method

Following conventional amplification with Normalase primers to generate libraries with indexed adapters to ≥12nM, a standard purification is performed. 4nM of each library is then enzymatically selected with Normalase I and the libraries are combined into a single pool. Next, Normalase II enzymatically normalises all libraries within the pool to 4nM and, without any need for further purification, the pool is ready for sequencing:

Normalase workflow

Libraries supported by Swift Normalase include those with full-length indexed adapters (e.g. Accel-NGS 2S, Swift 2S Turbo, KAPA®, Illumina®, TruSeq®), and libraries with an amplified yield of ≥12nM, or which have been prepared for direct sequencing (e.g. whole genome, whole transcriptome). By processing these with Swift Normalase, researchers can benefit from reduced variability, higher multiplexing per run, and fewer sample failures.

Amplification with Normalase primers is validated with multiple polymerases

 Amplification with Normalase primers is validated with multiple polymerases

A. Libraries generated with 50ng Coriell NA12878 DNA were amplified for 6 cycles with Normalase primers using three different DNA polymerases; NEB Q5 HiFi, Kapa® HiFi, and Swift HiFi (n=5 libraries/polymerase). All polymerases tested amplified libraries to ≥12nM, meeting the minimum threshold for Normalase. B. Amplified libraries were normalized with Normalase and sequenced on the Illumina® MiSeq using a V2 Mico flowcell 1x50 cycles. Performance of Normalase normalization was equivalent across the three HiFi polymerases. The variation across libraries was calculated at a CV of 8.7%.

Material available for download
Normalase - a novel library normalisation tool for high-throughput NGS
Normalase protocol
Normalase datasheet

Illumina® and TruSeq® are registered trademarks of Illumina, Inc.
KAPA® is a registered trademark of ROCHE MOLECULAR SYSTEMS, INC.

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