Swift Normalase Amplicon 16S & ITS1 NGS Panels (SNAP)

The Swift Normalase® Amplicon Panels (SNAP) Kit for 16S and ITS1 from Swift Bioscience, offers a robust NGS workflow that provides optimal coverage and NGS data quality on Illumina® sequencing platforms. This kit leverages Swift’s multiplex PCR technology, enabling library construction from DNA using tiled primer pairs to target either (a) all V1-V9 variable regions or (b) ITS1, each with a single pool of multiplexed primer pairs.

Swift Normalase Amplicon 16S & ITS1 NGS Panels (SNAP) utilise multiple overlapping amplicons in a single tube, using a rapid, 2-hour workflow to prepare ready-to-sequence libraries. The PCR1+PCR2 workflow generates robust libraries even from low input quantities of DNA that may be subsequently quantified and normalized with conventional methods such as Qubit® or Agilent Bioanalyzer, or optionally using the included Swift Normalase reagents.

Benefits of using the Swift Normalase Amplicon 16S & ITS1 NGS Panels (SNAP)
• Multiplexed assay covers all variable regions of the 16S rRNA or ITS1 genes in a single primer pool
• Integrated library normalization: Swift’s unique Normalase chemistry enables streamlined library balancing and pooling without the expense of quantification kits
• Unique amplicon chemistry generates diverse clusters without PhiX or phased primers, recovering >20% of reads
• Fast workflow: from DNA to libraries within 2 hours
• High quality data

Swift Normalase Amplicon 16S & ITS1 panel coverage
The Swift SNAP 16S and ITS1 Panels facilitate NGS analysis of complex microbial communities (e.g. bacteria, archaea, fungi) using a single primer pool targeting the 16S rRNA gene (variable regions 1-9) or ITS1 genes. In addition, these panels can be customized with additional targets including antibiotic resistance or virulence genes, allowing sub-genera level identification and functional analysis.

Sequencing read coverage (IGV Sashimi plot) and illustration of multiplexed primer coverage of all nine variable regions of 16S rRNA.

Specifications

Feature	SNAP 16S Panel or ITS1 Panel
Panel information	17 primers (16S) or 15 primers (ITS1); Average 425 bp amplicon size
Input material	1 ng bacterial or fungal template DNA
Time	2 hours cDNA-to-library or 3 hours cDNA-to-normalised-library-pool
Components provided	Target-specific multiplex primer pool PCR and library prep reagents Swift normalase Combinatorial dual indexed adapters Note: kits do not include magnetic bead
Multiplexing capability	Up to 384 CDI Enquire for custom indexing and UDIs
Recommended depth	100K reads per library

Material available to download
Swift Normalase Amplicon 16S & ITS1 NGS Panels (SNAP) protocol
Swift Normalase Amplicon 16S & ITS1 NGS Panels (SNAP) datasheet

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