www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

Swift Normalase Amplicon Panels (SNAP) 16S V2 and ITS1

The Swift Normalase® Amplicon Panels (SNAP) Kit for 16S and ITS1 from Swift Bioscience, offers a robust NGS workflow that provides optimal coverage and NGS data quality on Illumina® sequencing platforms. This kit leverages Swift’s multiplex PCR technology, enabling library construction from DNA using tiled primer pairs to target either (a) all V1-V9 variable regions or (b) ITS1, each with a single pool of multiplexed primer pairs.

Swift Normalase Amplicon 16S V2 & ITS1 NGS Panels (SNAP) utilise multiple overlapping amplicons in a single tube, using a rapid, 2-hour workflow to prepare ready-to-sequence libraries. The PCR1+PCR2 workflow generates robust libraries even from low input quantities of DNA that may be subsequently quantified and normalized with conventional methods such as Qubit® or Agilent Bioanalyzer, or optionally using the included Swift Normalase reagents.

Benefits of using the Swift Normalase Amplicon 16S V2 & ITS1 NGS Panels (SNAP)
• Multiplexed assay covers all variable regions of the 16S rRNA or ITS1 genes in a single primer pool
• Integrated library normalization: Swift’s unique Normalase chemistry enables streamlined library balancing and pooling without the expense of quantification kits
• Unique amplicon chemistry generates diverse clusters without PhiX or phased primers, recovering >20% of reads
• Fast workflow: from DNA to libraries within 2 hours
• High quality data

Swift Normalase Amplicon 16S V2 & ITS1 panel coverage
The Swift SNAP 16S and ITS1 Panels facilitate NGS analysis of complex microbial communities (e.g. bacteria, archaea, fungi) using a single primer pool targeting the 16S rRNA gene (variable regions 1-9) or ITS1 genes. In addition, these panels can be customized with additional targets including antibiotic resistance or virulence genes, allowing sub-genera level identification and functional analysis.

Swift 16S panel coverage

Sequencing read coverage (IGV Sashimi plot) and illustration of multiplexed primer coverage of all nine variable regions of 16S rRNA.

Specifications

Feature SNAP 16S V2 Panel or ITS1 Panel
Panel information 23 primers (16S), average 425 bp amplicon size  or 15 primers (ITS1), 145-695 bp amplicon size
Input material 10 pg for microbial isolates; 1-50 ng for metagenomic samples
Time 2 hours cDNA-to-library or 3 hours cDNA-to-normalised-library-pool
Components provided Target-specific multiplex primer pool
PCR and library prep reagents
Swift normalase
Combinatorial dual indexed adapters
Note: kits do not include magnetic bead
Multiplexing capability Up to 1536 CDI
Enquire for custom indexing and UDIs
Recommended depth 100K reads per library ITS1, 25K reads per library 16S v2


Material available to download
Swift Normalase Amplicon 16S & ITS1 NGS Panels (SNAP) protocol
Swift Normalase Amplicon 16S & ITS1 NGS Panels (SNAP) datasheet

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
16S Panel v2 (primers only) 516G1-96 · Swift Biosciences
516G1-96
Swift Biosciences
1 each £383.00
1 each
view
ITS1 Panel (primers only) 517G1-96 · Swift Biosciences
517G1-96
Swift Biosciences
Each £383.00
Each
view
Swift Normalase┬« Amplicon Panels (SNAP) Core Kit SN-5X296 · Swift Biosciences
SN-5X296
Swift Biosciences
Each £2318.00
Each
view
SNAP Set 1A Combinatorial Dual Indexing Primers SN-5S1A96 · Swift Biosciences
SN-5S1A96
Swift Biosciences
96 reactions £597.00
96 reactions
view
SNAP Set 1B Combinatorial Dual Indexing Primers SN-5S1B96 · Swift Biosciences
SN-5S1B96
Swift Biosciences
96 reactions £597.00
96 reactions
view
SNAP Set 2A Combinatorial Dual Indexing Primers SN-5S2A96 · Swift Biosciences
SN-5S2A96
Swift Biosciences
96 reactions £597.00
96 reactions
view
SNAP Set 2B Combinatorial Dual Indexing Primers SN-5S2B96 · Swift Biosciences
SN-5S2B96
Swift Biosciences
96 reactions £597.00
96 reactions
view
Swift Normalase┬« Amplicon Panels (SNAP) Core Kit SN-5X384 · Swift Biosciences
SN-5X384
Swift Biosciences
Each £8713.00
Each
view
SNAP Set S1AB-S2AB Combinatorial Dual Indexing Primers SN-5S0384 · Swift Biosciences
SN-5S0384
Swift Biosciences
4 x 96 reactions £2387.00
4 x 96 reactions
view