The Swift Normalase® Amplicon Panels (SNAP) Kit for 16S and ITS1 from Swift Bioscience, offers a robust NGS workflow that provides optimal coverage and NGS data quality on Illumina® sequencing platforms. This kit leverages Swift’s multiplex PCR technology, enabling library construction from DNA using tiled primer pairs to target either (a) all V1-V9 variable regions or (b) ITS1, each with a single pool of multiplexed primer pairs.
Swift Normalase Amplicon 16S & ITS1 NGS Panels (SNAP) utilise multiple overlapping amplicons in a single tube, using a rapid, 2-hour workflow to prepare ready-to-sequence libraries. The PCR1+PCR2 workflow generates robust libraries even from low input quantities of DNA that may be subsequently quantified and normalized with conventional methods such as Qubit® or Agilent Bioanalyzer, or optionally using the included Swift Normalase reagents.
Benefits of using the Swift Normalase Amplicon 16S & ITS1 NGS Panels (SNAP)
• Multiplexed assay covers all variable regions of the 16S rRNA or ITS1 genes in a single primer pool
• Integrated library normalization: Swift’s unique Normalase chemistry enables streamlined library balancing and pooling without the expense of quantification kits
• Unique amplicon chemistry generates diverse clusters without PhiX or phased primers, recovering >20% of reads
• Fast workflow: from DNA to libraries within 2 hours
• High quality data
Swift Normalase Amplicon 16S & ITS1 panel coverage
The Swift SNAP 16S and ITS1 Panels facilitate NGS analysis of complex microbial communities (e.g. bacteria, archaea, fungi) using a single primer pool targeting the 16S rRNA gene (variable regions 1-9) or ITS1 genes. In addition, these panels can be customized with additional targets including antibiotic resistance or virulence genes, allowing sub-genera level identification and functional analysis.
Sequencing read coverage (IGV Sashimi plot) and illustration of multiplexed primer coverage of all nine variable regions of 16S rRNA.
Specifications
| Feature | SNAP 16S Panel or ITS1 Panel |
|---|---|
| Panel information | 17 primers (16S) or 15 primers (ITS1); Average 425 bp amplicon size |
| Input material | 1 ng bacterial or fungal template DNA |
| Time | 2 hours cDNA-to-library or 3 hours cDNA-to-normalised-library-pool |
| Components provided | Target-specific multiplex primer pool PCR and library prep reagents Swift normalase Combinatorial dual indexed adapters Note: kits do not include magnetic bead |
| Multiplexing capability | Up to 384 CDI Enquire for custom indexing and UDIs |
| Recommended depth | 100K reads per library |
Material available to download
Swift Normalase Amplicon 16S & ITS1 NGS Panels (SNAP) protocol
Swift Normalase Amplicon 16S & ITS1 NGS Panels (SNAP) datasheet
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| 16S Panel v2 (primers only) 516G1-96 · Swift Biosciences | 516G1-96 Swift Biosciences |
96 libraries |
£371.00
96 libraries
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| ITS1 Panel (primers only) 517G1-96 · Swift Biosciences | 517G1-96 Swift Biosciences |
96 libraries |
£371.00
96 libraries
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|
| Swift Normalase Amplicon Protocol SNAP Core (no indexing) SN-5X296 · Swift Biosciences | SN-5X296 Swift Biosciences |
96 reactions |
£2318.00
96 reactions
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|
| SNAP Combinatorial Dual Index Primer Kit (Set 1A) SN-5S1A96 · Swift Biosciences | SN-5S1A96 Swift Biosciences |
96 reactions |
£580.00
96 reactions
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|
| SNAP Combinatorial Dual Index Primer Kit (Set 1B) SN-5S1B96 · Swift Biosciences | SN-5S1B96 Swift Biosciences |
96 reactions |
£580.00
96 reactions
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|
| SNAP Combinatorial Dual Index Primer Kit (Set 2A) SN-5S2A96 · Swift Biosciences | SN-5S2A96 Swift Biosciences |
96 reactions |
£580.00
96 reactions
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|
| SNAP Combinatorial Dual Index Primer Kit (Set 2B) SN-5S2B96 · Swift Biosciences | SN-5S2B96 Swift Biosciences |
96 reactions |
£580.00
96 reactions
view
|
