The Swift Normalase® Amplicon Panels (SNAP) Kit for 16S and ITS1 from Swift Bioscience, offers a robust NGS workflow that provides optimal coverage and NGS data quality on Illumina® sequencing platforms. This kit leverages Swift’s multiplex PCR technology, enabling library construction from DNA using tiled primer pairs to target either (a) all V1-V9 variable regions or (b) ITS1, each with a single pool of multiplexed primer pairs.
Swift Normalase Amplicon 16S V2 & ITS1 NGS Panels (SNAP) utilise multiple overlapping amplicons in a single tube, using a rapid, 2-hour workflow to prepare ready-to-sequence libraries. The PCR1+PCR2 workflow generates robust libraries even from low input quantities of DNA that may be subsequently quantified and normalized with conventional methods such as Qubit® or Agilent Bioanalyzer, or optionally using the included Swift Normalase reagents.
Benefits of using the Swift Normalase Amplicon 16S V2 & ITS1 NGS Panels (SNAP)
• Multiplexed assay covers all variable regions of the 16S rRNA or ITS1 genes in a single primer pool
• Integrated library normalization: Swift’s unique Normalase chemistry enables streamlined library balancing and pooling without the expense of quantification kits
• Unique amplicon chemistry generates diverse clusters without PhiX or phased primers, recovering >20% of reads
• Fast workflow: from DNA to libraries within 2 hours
• High quality data
Swift Normalase Amplicon 16S V2 & ITS1 panel coverage
The Swift SNAP 16S and ITS1 Panels facilitate NGS analysis of complex microbial communities (e.g. bacteria, archaea, fungi) using a single primer pool targeting the 16S rRNA gene (variable regions 1-9) or ITS1 genes. In addition, these panels can be customized with additional targets including antibiotic resistance or virulence genes, allowing sub-genera level identification and functional analysis.
Sequencing read coverage (IGV Sashimi plot) and illustration of multiplexed primer coverage of all nine variable regions of 16S rRNA.
Specifications
Feature | SNAP 16S V2 Panel or ITS1 Panel |
---|---|
Panel information | 23 primers (16S), average 425 bp amplicon size or 15 primers (ITS1), 145-695 bp amplicon size |
Input material | 10 pg for microbial isolates; 1-50 ng for metagenomic samples |
Time | 2 hours cDNA-to-library or 3 hours cDNA-to-normalised-library-pool |
Components provided | Target-specific multiplex primer pool PCR and library prep reagents Swift normalase Combinatorial dual indexed adapters Note: kits do not include magnetic bead |
Multiplexing capability | Up to 1536 CDI Enquire for custom indexing and UDIs |
Recommended depth | 100K reads per library ITS1, 25K reads per library 16S v2 |
Material available to download
Swift Normalase Amplicon 16S & ITS1 NGS Panels (SNAP) protocol
Swift Normalase Amplicon 16S & ITS1 NGS Panels (SNAP) datasheet
Products
Note: product availability depends on country - see product detail page.
Details | Cat number & supplier | Size | Price |
16S Panel v2 (primers only) 516G1-96 · Swift Biosciences | 516G1-96 Swift Biosciences |
1 each |
£383.00
1 each
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ITS1 Panel (primers only) 517G1-96 · Swift Biosciences | 517G1-96 Swift Biosciences |
Each |
£383.00
Each
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Swift Normalase® Amplicon Panels (SNAP) Core Kit SN-5X296 · Swift Biosciences | SN-5X296 Swift Biosciences |
Each |
£2318.00
Each
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SNAP Set 1A Combinatorial Dual Indexing Primers SN-5S1A96 · Swift Biosciences | SN-5S1A96 Swift Biosciences |
96 reactions |
£597.00
96 reactions
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SNAP Set 1B Combinatorial Dual Indexing Primers SN-5S1B96 · Swift Biosciences | SN-5S1B96 Swift Biosciences |
96 reactions |
£597.00
96 reactions
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SNAP Set 2A Combinatorial Dual Indexing Primers SN-5S2A96 · Swift Biosciences | SN-5S2A96 Swift Biosciences |
96 reactions |
£597.00
96 reactions
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SNAP Set 2B Combinatorial Dual Indexing Primers SN-5S2B96 · Swift Biosciences | SN-5S2B96 Swift Biosciences |
96 reactions |
£597.00
96 reactions
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Swift Normalase® Amplicon Panels (SNAP) Core Kit SN-5X384 · Swift Biosciences | SN-5X384 Swift Biosciences |
Each |
£8713.00
Each
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SNAP Set S1AB-S2AB Combinatorial Dual Indexing Primers SN-5S0384 · Swift Biosciences | SN-5S0384 Swift Biosciences |
4 x 96 reactions |
£2387.00
4 x 96 reactions
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