Amplicon SARS-CoV-2 NGS assay

The Swift Amplicon SARS-CoV-2 Panel from Swift Biosciences enables library construction from 1st strand and 2nd strand cDNA using tiled primer pairs, targeting the entire 29.9 kb SARS-CoV2 genome with a single pool of multiplexed pairs. Utlising patented multiplex PCR construction and primers designed against the NCBI Reference Sequence NC_045512.2 (severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome), this panel offers a robust NGS workflow that provides optimal coverage and NGS data quality on Illumina® sequencing platforms with zero predicted off-target products (determined by in silico analysis) from host genome sequences.

Benefits of using the Swift Amplicon SARS-CoV-2 Panel
• Library construction from 1st-strand or 2nd-strand cDNA
• Targets the entire 29.9 kb viral genome with a single pool of multiplexed primer pairs
• Primers designed against the NCBI Reference Sequence NC_045512.2
• Patented multiplex PCR technology
• Zero off-target products predicted (in silico analysis) from human host genome sequences.Swift Amplicon SARS-CoV-2 Research Panel workflow

Feature Specification
Design coverage 98% (29,313 of 29,903 total bases)
Panel information 341 amplicons, sized 116-255 bp (average 150 bp)
Input material 1st or 2nd strand cDNA
Time 2 hours cDNA-to-library
Components provided Target-specific multiplex primer pool
PCR and library prep reagents
8 x 12 Combinatorial Dual Indexed Adapters
Note: kit does not include RT module or magnetic beads
Multiplexing capability 96 included
Up to 384 possible (Each kit enables multiplexing 48 unique libraries. Purchase 2 kits to multiplex 96 samples. Combine with AL-S1A96, AL-S1B96, AL-S2A96 and/or AL-S2B96 to multiplex up to 384 samples)
Recommended depth 1M reads per library


Material available for download
Swift Amplicon SARS-CoV-2 Research Panel technical note

Illumina is a registered trademark of Illumina, Inc.

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Swift Amplicon SARS-CoV-2 Panel AL-COV48 · Swift Biosciences
AL-COV48
Swift Biosciences
48 reactions £2582.00
48 reactions
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