The Swift Amplicon SARS-CoV-2 Panel from Swift Biosciences enables library construction from 1st strand and 2nd strand cDNA using tiled primer pairs, targeting the entire 29.9 kb SARS-CoV2 genome with a single pool of multiplexed pairs. Utlising patented multiplex PCR construction and primers designed against the NCBI Reference Sequence NC_045512.2 (severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome), this panel offers a robust NGS workflow that provides optimal coverage and NGS data quality on Illumina® sequencing platforms with zero predicted off-target products (determined by in silico analysis) from host genome sequences.
Benefits of using the Swift Amplicon SARS-CoV-2 Panel
• Library construction from 1st-strand or 2nd-strand cDNA
• Targets the entire 29.9 kb viral genome with a single pool of multiplexed primer pairs
• Primers designed against the NCBI Reference Sequence NC_045512.2
• Patented multiplex PCR technology
• Zero off-target products predicted (in silico analysis) from human host genome sequences.
| Feature | Specification |
|---|---|
| Design coverage | 98% (29,313 of 29,903 total bases) |
| Panel information | 341 amplicons, sized 116-255 bp (average 150 bp) |
| Input material | 1st or 2nd strand cDNA |
| Time | 2 hours cDNA-to-library |
| Components provided | Target-specific multiplex primer pool PCR and library prep reagents 8 x 12 Combinatorial Dual Indexed Adapters Note: kit does not include RT module or magnetic beads |
| Multiplexing capability | 96 included Up to 384 possible (Each kit enables multiplexing 48 unique libraries. Purchase 2 kits to multiplex 96 samples. Combine with AL-S1A96, AL-S1B96, AL-S2A96 and/or AL-S2B96 to multiplex up to 384 samples) |
| Recommended depth | 1M reads per library |
Material available for download
Swift Amplicon SARS-CoV-2 Research Panel technical note
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