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StemTAG™ Stem Cell Colony Formation Assay

StemTAG™ Stem Cell Colony Formation Assay enables accurate and high-throughput quantification of stem cell colonies in just 7-10 days with no manual cell counting required. The short incubation time makes assaying transiently transfected cells possible. Using a solubilisation media and CyQuant™ dye to detect cell numbers fluorometrically or colourimetrically, viable colony-forming cells (CFCs) can alternatively be recovered for further culturing and testing. The kit provides quantities to perform 96 tests in a microtitre plate but can be modified for use in other plate formats (see the protocol in material available for download).

Benefits of using StemTAG™ Stem Cell Colony Formation Assay
• Results in only 7-10 days
• Simple process
• Accurate quantification of stem cell colony formation
• No manual cell counting
• Easy viable cell recovery of colony forming cells (CFCs)
• High-throughput compared to homebrew techniques
• Colourimetric and fluorometric detection options
• Assay transiently transfected cells

StemTag™ Stem Cell Colony Formation Assay principle

Cells may be either lysed and quantified or recovered for further downstream analysis.

StemTag™ stem cell colony formation
Anchorage-independent growth of mouse ES-D3 cells. Alkaline phosphatase staining.

StemTAG™ Stem Cell Colony Formation Assay citations

Material available for download

StemTAG™ Stem Cell Colony Formation Assay (cell recovery compatible) protocol

CyQuant™ is a trademark of Molecular Probes.

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
StemTAG™ Stem Cell Colony Formation Assay (Cell Recovery Compatible) CBA-325 · Cell Biolabs
CBA-325
Cell Biolabs
96 assays £934.00
96 assays
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StemTAG™ Stem Cell Colony Formation Assay (Cell Recovery Compatible) CBA-325-5 · Cell Biolabs
CBA-325-5
Cell Biolabs
5 x 96 assays £3841.00
5 x 96 assays
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