Stem Cell Retroviral Expression System

Cambridge Bioscience offers the a range of retroviral expression systems for the transduction of embryonic stem cells (ESC), embryonic carcinoma cells (ECC) and haematopoietic stem cells (HSC). Utilising both superior packaging cell lines and vector technologies to produce high-titre virus with a single plasmid transfection, our range of kits are available for amphotropic, ecotropic and pantropic virus particles production.

Benefits of the Stem Cell Retroviral Expression System
• High Titre - 10⁷ infectious units/mL average titer with transient transfection
• Ecotropic, amphotropic or pantropic retrovirus produced
• Optimised for expression in either ES/EC cells or HSC's

Kit Components
Each platinum retroviral expression system kit includes the following components:

1. Exclusive Platinum Packaging Cell Line
- Plat-E (ecotropic, Plat-A (amphotropic), or Plat-GP (pantropic) packaging lines are included with their respective kits.
- Each cell line contains the gag and pol genes
- Plat-E and Plat-A cells contain the envelope protein
- Plat-GP must be co-transfected with the VSV-G plasmid included in the kit

2. Expression Vectors
- pMCs-Puro expression vector included in the ESC/ECC kits
- pMYs-Puro expression vector included in the HSC kits
-Vectors provide the viral package signal, transcription and processing elements and MCS for cloning of the target gene

3. GFP Control Vectors
- pMCs-GFP or pMYs-GFP control vector contains the ampicillin-resistance gene, LTRs, package signal and GFP insert

Material Available for Download
Amphotropic Retroviral Expression System for ES/EC Cells User Manual
Amphotropic Retroviral Expression System for Hematopoietic Stem Cells User Manual
Ecotropic Retroviral Expression System for ES/EC Cells User Manual
Ecotropic Retroviral Expression System for Hematopoietic Stem Cells User Manual
Pantropic Retroviral Expression System for ES/EC Cells User Manual
Pantropic Retroviral Expression System for Hematopoietic Stem Cells User Manual