Cambridge Bioscience offers a range of lentiviral expression vectors for the effective delivery and stable expression of any cDNA in a wide range of mammalian cells. With a wide selection of promoter options available including CMV (Cytomegalovirus), MSCV (Murine Stem Cell Virus), UbC (Ubiquitin C), PGK (Phosphoglycerate Kinase) and EF1 (Elongation Factor 1α), deliver your cDNA construct of interest to both dividing and non-dividing cells.
Benefits of Using These cDNA Lentivectors• Reliable delivery to dividing or non-dividing cells
• Biosafe
• Single, double and bidirectional promoters available
• GFP, RFP, Puro, Neo or Hygro options
• HIV or FIV-based
Gene Expression from Constitutive Promoters
1. Single Promoter Vectors
Characterised by the presence of a single mammalian promoter driving the gene on interest cloned into the MCS, either expressed by itself or co-expressed with a marker gene of interest (e.g. copGFP or Puro).
2. Dual Promoter Vectors
Characterised by the presence of two independent mammalian promoters, one driving the gene of interest cloned into the MCS & the other driving the expression of a marker (e.g. copGFP, Puro, or GFP-T2A-Puro). These vectors are typically used to assess transduction efficiency or generate stably selected cell lines independent of the gene of interest.
3. Bidirectional Promoter Vectors
We offer a synthetic bidirectional promoter with EF1 & PGK in a divergent configuration coordinating the regulation of two or more different transgenes. Coordinate dual expression of your gene of interest as well as various markers (GFP, RFP, Puro, etc.) without the use of IRES elements which have weak functions when co-expressing two cDNA.
Find Out More
To request further information, contact our System Biosciences Specialist Here
Custom Lentiviral Cloning Service
Find out more about our range of custom lentiviral cloning services that we offer Here
Material Available for Download
pCDH cDNA Cloning & Expression Lentivectors Manual
pCDF cDNA Cloning & Expression Lentivectors Manual
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector CD810A-1 · System Biosciences | CD810A-1 System Biosciences |
10 µg |
£539.00
10 µg
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| pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector CD811A-1 · System Biosciences | CD811A-1 System Biosciences |
10 µg |
£539.00
10 µg
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| pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector CD813A-1 · System Biosciences | CD813A-1 System Biosciences |
10 µg |
£539.00
10 µg
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| pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector CD822A-1 · System Biosciences | CD822A-1 System Biosciences |
10 µg |
£539.00
10 µg
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| pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector CD823A-1 · System Biosciences | CD823A-1 System Biosciences |
10 µg |
£539.00
10 µg
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| pCDF1-MCS1 cDNA Cloning and Expression Vector CD100A-1 · System Biosciences | CD100A-1 System Biosciences |
10 µg |
£450.00
10 µg
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| pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector CD110B-1 · System Biosciences | CD110B-1 System Biosciences |
10 µg |
£532.00
10 µg
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| pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector CD111B-1 · System Biosciences | CD111B-1 System Biosciences |
10 µg |
£532.00
10 µg
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| pCDH-CMV-MCS cDNA Cloning and Expression Vector CD500B-1 · System Biosciences | CD500B-1 System Biosciences |
10 µg |
£450.00
10 µg
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| pCDH-CMV-MCS2 cDNA Cloning and Expression Vector CD501A-1 · System Biosciences | CD501A-1 System Biosciences |
10 µg |
£450.00
10 µg
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| pCDH-EF1-MCS cDNA Cloning and Expression Vector CD502A-1 · System Biosciences | CD502A-1 System Biosciences |
10 µg |
£450.00
10 µg
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| pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector CD511B-1 · System Biosciences | CD511B-1 System Biosciences |
10 µg |
£532.00
10 µg
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| pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector CD512B-1 · System Biosciences | CD512B-1 System Biosciences |
10 µg |
£532.00
10 µg
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| pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector CD513B-1 · System Biosciences | CD513B-1 System Biosciences |
10 µg |
£532.00
10 µg
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| pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector CD514B-1 · System Biosciences | CD514B-1 System Biosciences |
10 µg |
£532.00
10 µg
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| pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector CD515B-1 · System Biosciences | CD515B-1 System Biosciences |
10 µg |
£532.00
10 µg
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| pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector CD516B-2 · System Biosciences | CD516B-2 System Biosciences |
10 µg |
£532.00
10 µg
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| pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector CD522A-1 · System Biosciences | CD522A-1 System Biosciences |
10 µg |
£548.00
10 µg
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| pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector CD523A-1 · System Biosciences | CD523A-1 System Biosciences |
10 µg |
£548.00
10 µg
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| pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector CD524A-1 · System Biosciences | CD524A-1 System Biosciences |
10 µg |
£548.00
10 µg
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| pCDH-EF1-MCS*-T2A-GFP CD526A-1 · System Biosciences | CD526A-1 System Biosciences |
10 µg |
£548.00
10 µg
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| pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector CD527A-1 · System Biosciences | CD527A-1 System Biosciences |
10 µg |
£548.00
10 µg
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| pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector CD530A-2 · System Biosciences | CD530A-2 System Biosciences |
10 µg |
£548.00
10 µg
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| pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector CD531A-2 · System Biosciences | CD531A-2 System Biosciences |
10 µg |
£548.00
10 µg
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| pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector CD532A-2 · System Biosciences | CD532A-2 System Biosciences |
10 µg |
£548.00
10 µg
view
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