Stable Over-Expression with cDNA Lentivectors

Cambridge Bioscience offers a range of lentiviral expression vectors for the effective delivery and stable expression of any cDNA in a wide range of mammalian cells. With a wide selection of promoter options available including CMV (Cytomegalovirus), MSCV (Murine Stem Cell Virus), UbC (Ubiquitin C), PGK (Phosphoglycerate Kinase) and EF1 (Elongation Factor 1α), deliver your cDNA construct of interest to both dividing and non-dividing cells.

Benefits of Using These cDNA Lentivectors
• Reliable delivery to dividing or non-dividing cells
• Biosafe
• Single, double and bidirectional promoters available
• GFP, RFP, Puro, Neo or Hygro options
• HIV or FIV-based

Gene Expression from Constitutive Promoters


1. Single Promoter Vectors
Characterised by the presence of a single mammalian promoter driving the gene on interest cloned into the MCS, either expressed by itself or co-expressed with a marker gene of interest (e.g. copGFP or Puro).
2. Dual Promoter Vectors
Characterised by the presence of two independent mammalian promoters, one driving the gene of interest cloned into the MCS & the other driving the expression of a marker (e.g. copGFP, Puro, or GFP-T2A-Puro). These vectors are typically used to assess transduction efficiency or generate stably selected cell lines independent of the gene of interest.
3. Bidirectional Promoter Vectors
We offer a synthetic bidirectional promoter with EF1 & PGK in a divergent configuration coordinating the regulation of two or more different transgenes. Coordinate dual expression of your gene of interest as well as various markers (GFP, RFP, Puro, etc.) without the use of IRES elements which have weak functions when co-expressing two cDNA.

Find Out More
To request further information, contact our System Biosciences Specialist Here

Custom Lentiviral Cloning Service

Find out more about our range of custom lentiviral cloning services that we offer Here

Material Available for Download
pCDH cDNA Cloning & Expression Lentivectors Manual
pCDF cDNA Cloning & Expression Lentivectors Manual

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector CD810A-1 · System Biosciences
CD810A-1
System Biosciences
10 µg £528.00
10 µg
view
pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector CD811A-1 · System Biosciences
CD811A-1
System Biosciences
10 µg £528.00
10 µg
view
pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector CD813A-1 · System Biosciences
CD813A-1
System Biosciences
10 µg £528.00
10 µg
view
pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector CD822A-1 · System Biosciences
CD822A-1
System Biosciences
10 µg £528.00
10 µg
view
pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector CD823A-1 · System Biosciences
CD823A-1
System Biosciences
10 µg £528.00
10 µg
view
pCDF1-MCS1 cDNA Cloning and Expression Vector CD100A-1 · System Biosciences
CD100A-1
System Biosciences
10 µg £441.00
10 µg
view
pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector CD110B-1 · System Biosciences
CD110B-1
System Biosciences
10 µg £521.00
10 µg
view
pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector CD111B-1 · System Biosciences
CD111B-1
System Biosciences
10 µg £521.00
10 µg
view
pCDH-CMV-MCS cDNA Cloning and Expression Vector CD500B-1 · System Biosciences
CD500B-1
System Biosciences
10 µg £441.00
10 µg
view
pCDH-CMV-MCS2 cDNA Cloning and Expression Vector CD501A-1 · System Biosciences
CD501A-1
System Biosciences
10 µg £441.00
10 µg
view
pCDH-EF1-MCS cDNA Cloning and Expression Vector CD502A-1 · System Biosciences
CD502A-1
System Biosciences
10 µg £441.00
10 µg
view
pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector CD511B-1 · System Biosciences
CD511B-1
System Biosciences
10 µg £521.00
10 µg
view
pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector CD512B-1 · System Biosciences
CD512B-1
System Biosciences
10 µg £521.00
10 µg
view
pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector CD513B-1 · System Biosciences
CD513B-1
System Biosciences
10 µg £521.00
10 µg
view
pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector CD514B-1 · System Biosciences
CD514B-1
System Biosciences
10 µg £521.00
10 µg
view
pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector CD515B-1 · System Biosciences
CD515B-1
System Biosciences
10 µg £521.00
10 µg
view
pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector CD516B-2 · System Biosciences
CD516B-2
System Biosciences
10 µg £521.00
10 µg
view
pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector CD522A-1 · System Biosciences
CD522A-1
System Biosciences
10 µg £538.00
10 µg
view
pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector CD523A-1 · System Biosciences
CD523A-1
System Biosciences
10 µg £538.00
10 µg
view
pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector CD524A-1 · System Biosciences
CD524A-1
System Biosciences
10 µg £538.00
10 µg
view
pCDH-EF1-MCS*-T2A-GFP CD526A-1 · System Biosciences
CD526A-1
System Biosciences
10 µg £538.00
10 µg
view
pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 ug CD527A-1 · System Biosciences
CD527A-1
System Biosciences
10 µg £538.00
10 µg
view
pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector CD530A-2 · System Biosciences
CD530A-2
System Biosciences
10 µg £538.00
10 µg
view
pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector CD531A-2 · System Biosciences
CD531A-2
System Biosciences
10 µg £538.00
10 µg
view
pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector CD532A-2 · System Biosciences
CD532A-2
System Biosciences
10 µg £538.00
10 µg
view