Sensitive & Specific 5-mC DNA Quantification Kit

Cambridge Bioscience offers a sensitive and specific 5-methylcytosine (5-mC) DNA ELISA kit for the global quantification of 5-mC in DNA. Using a unique anti-5-methylcytosine monoclonal antibody that is both sensitive and specific for 5-mC, quantify 5-mC from a wide range of sample types in less than 3 hours.

Benefits of Using the 5-mC DNA ELISA Kit
• Sensitive
• 5-mC specific
• < 3 hour protocol
• Ideal for high-throughput analyses

Comparison of 5-mC DNA ELISA Kit Data with Mass Spectrometry Data
 

The 5-mC DNA ELISA Kit can quantify 5-mC in numerous DNA samples with close correlation to LC-MS/MS-MRM analysis. 100 ng of genomic DNA from human brain (HB), human kidney (HK), human embryonic stem cell (HESC), mouse brain (MB), mouse kidney (MK), mouse testes (MT), HCT116 double knock-out (DKO), and M.SssI treated HCT116 DKO (DKO+CpG) was used to coat wells, in triplicate. Percent 5-mC was calculated using the second-order regression equation of the standard curve that was constructed with the negative and positive control.

Material Available for Download
5-mC DNA ELISA Kit Datasheet
5-mC DNA ELISA Kit Protocol

5-mC DNA ELISA Kit Citation List

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
5-mC DNA ELISA Kit D5325 · Zymo Research
D5325
Zymo Research
96 wells £501.00
96 wells
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5-mC DNA ELISA Kit D5326 · Zymo Research
D5326
Zymo Research
2 x 96 wells £799.00
2 x 96 wells
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