Benefits of Using the 5-mC DNA ELISA Kit
• 5-mC specific
• < 3 hour protocol
• Ideal for high-throughput analyses
Comparison of 5-mC DNA ELISA Kit Data with Mass Spectrometry Data
The 5-mC DNA ELISA Kit can quantify 5-mC in numerous DNA samples with close correlation to LC-MS/MS-MRM analysis. 100 ng of genomic DNA from human brain (HB), human kidney (HK), human embryonic stem cell (HESC), mouse brain (MB), mouse kidney (MK), mouse testes (MT), HCT116 double knock-out (DKO), and M.SssI treated HCT116 DKO (DKO+CpG) was used to coat wells, in triplicate. Percent 5-mC was calculated using the second-order regression equation of the standard curve that was constructed with the negative and positive control.
Material Available for Download
5-mC DNA ELISA Kit Datasheet
5-mC DNA ELISA Kit Protocol
5-mC DNA ELISA Kit Citation List
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